Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.184 extracted from

  • Gonzalez-Covarrubias, V.; Ghosh, D.; Lakhman, S.S.; Pendyala, L.; Blanco, J.G.
    A functional genetic polymorphism on human carbonyl reductase 1 (CBR1 V88I) impacts on catalytic activity and NADPH binding affinity (2007), Drug Metab. Dispos., 35, 973-980.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
V88I functional genetic polymorphism. Mutation results in CBR1 isoforms with different binding affinities for cofactor NADPH and inhibitor rutin as well as different maximal velocities for reaction with daunorubicin and prostaglandin E2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 menadione wild-type, 37°C, pH 7.4 Homo sapiens
0.055
-
 menadione mutant V88I, 37°C, pH 7.4 Homo sapiens
0.14
-
 daunorubicin mutant V88I, 37°C, pH 7.4 Homo sapiens
0.173
-
 daunorubicin wild-type, 37°C, pH 7.4 Homo sapiens
0.247
-
 NADPH wild-type, 37°C, pH 7.4 Homo sapiens
0.309
-
 prostaglandin E2 wild-type, 37°C, pH 7.4 Homo sapiens
0.32
-
 prostaglandin E2 mutant V88I, 37°C, pH 7.4 Homo sapiens
0.343
-
 NADPH mutant V88I, 37°C, pH 7.4 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform CBR1
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daunorubicin + NADPH
-
 Homo sapiens daunorubicinol + NADP+
-
 ?
menadione + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
prostaglandin E2 + NADPH
-
 Homo sapiens prostaglandin F2alpha + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18
-
 prostaglandin E2 mutant V88I, 37°C, pH 7.4 Homo sapiens
25
-
 prostaglandin E2 wild-type, 37°C, pH 7.4 Homo sapiens
61
-
 daunorubicin mutant V88I, 37°C, pH 7.4 Homo sapiens
85
-
 menadione mutant V88I, 37°C, pH 7.4 Homo sapiens
91
-
 daunorubicin wild-type, 37°C, pH 7.4 Homo sapiens
110
-
 menadione wild-type, 37°C, pH 7.4 Homo sapiens
149
-
 NADPH mutant V88I, 37°C, pH 7.4 Homo sapiens
179
-
 NADPH wild-type, 37°C, pH 7.4 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Homo sapiens
NADPH
-
 Homo sapiens