Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V88I | functional genetic polymorphism. Mutation results in CBR1 isoforms with different binding affinities for cofactor NADPH and inhibitor rutin as well as different maximal velocities for reaction with daunorubicin and prostaglandin E2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
menadione | wild-type, 37°C, pH 7.4 | Homo sapiens | |
0.055 | - |
menadione | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
0.14 | - |
daunorubicin | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
0.173 | - |
daunorubicin | wild-type, 37°C, pH 7.4 | Homo sapiens | |
0.247 | - |
NADPH | wild-type, 37°C, pH 7.4 | Homo sapiens | |
0.309 | - |
prostaglandin E2 | wild-type, 37°C, pH 7.4 | Homo sapiens | |
0.32 | - |
prostaglandin E2 | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
0.343 | - |
NADPH | mutant V88I, 37°C, pH 7.4 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform CBR1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
daunorubicin + NADPH | - |
Homo sapiens | daunorubicinol + NADP+ | - |
? | |
menadione + NADPH | - |
Homo sapiens | ? + NADP+ | - |
? | |
prostaglandin E2 + NADPH | - |
Homo sapiens | prostaglandin F2alpha + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
prostaglandin E2 | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
25 | - |
prostaglandin E2 | wild-type, 37°C, pH 7.4 | Homo sapiens | |
61 | - |
daunorubicin | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
85 | - |
menadione | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
91 | - |
daunorubicin | wild-type, 37°C, pH 7.4 | Homo sapiens | |
110 | - |
menadione | wild-type, 37°C, pH 7.4 | Homo sapiens | |
149 | - |
NADPH | mutant V88I, 37°C, pH 7.4 | Homo sapiens | |
179 | - |
NADPH | wild-type, 37°C, pH 7.4 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |