Cloned (Comment) | Organism |
---|---|
expression of the heart enzyme in Escherichia coli BL21(DE3), assembly to tetrameric form | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
hexaphenone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.05 | - |
valerophenone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.09 | - |
1,4-Naphthoquinone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.09 | - |
pyridin-4-yl hexanoate | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.16 | - |
1,4-benzoquinone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.17 | - |
n-butyrophenone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.2 | - |
4-nitrobenzaldehyde | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.29 | - |
menadione | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.29 | - |
4-benzoylpyridine | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.76 | - |
3-benzoylpyridine | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.89 | - |
propiophenone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
0.93 | - |
2-benzoylpyridine | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
1.2 | - |
4-Nitroacetophenone | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
1.4 | - |
Pyridine-4-aldehyde | pH 6.0, 25°C, recombinant enzyme | Sus scrofa | |
11.4 | - |
Pyridine-3-aldehyde | pH 6.0, 25°C, recombinant enzyme | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADPH | native and recombinant enzymes, low activity | Sus scrofa | ? + NADP+ | - |
? | |
1,4-naphthoquinone + NADPH + H+ | native and recombinant enzymes, low activity | Sus scrofa | ? + NADP+ | - |
? | |
2-benzoylpyridine + NADPH | highly stereoselective reaction, native and recombinant enzymes, low activity | Sus scrofa | (S)-alpha-phenyl-2-pyridylmethanol + NADP+ | - |
? | |
3-benzoylpyridine + NADPH | highly stereoselective reaction, native and recombinant enzymes | Sus scrofa | (S)-alpha-phenyl-3-pyridylmethanol + NADP+ | - |
? | |
4-benzoylpyridine + NADPH + H+ | highly stereoselective reaction, native and recombinant enzymes | Sus scrofa | (S)-alpha-phenyl-4-pyridylmethanol + NADP+ | - |
? | |
4-hexanoylpyridine + NADPH + H+ | native and recombinant enzymes | Sus scrofa | 1-(pyridin-4-yloxy)hexan-1-ol + NADP+ | - |
? | |
4-nitroacetophenone + NADPH | native and recombinant enzymes, low activity | Sus scrofa | 1-(4-nitrophenyl)ethanol + NADP+ | - |
? | |
4-nitrobenzaldehyde + NADPH | native and recombinant enzymes | Sus scrofa | 4-nitrobenzylalcohol + NADP+ | - |
? | |
hexaphenone + NADPH | native and recombinant enzymes | Sus scrofa | 1-phenylhexan-1-ol + NADP+ | - |
? | |
menadione + NADPH | native and recombinant enzymes, low activity | Sus scrofa | ? + NADP+ | - |
? | |
additional information | substrate specificity, no activity with 3-nitroacetophenone and 4-acetylpyridine, catalytic activity of the recombinant enzyme is similar to the native enzyme | Sus scrofa | ? | - |
? | |
n-butyrophenone + NADPH | native and recombinant enzymes, low activity | Sus scrofa | 1-phenylbutan-1-ol + NADP+ | - |
? | |
propiophenone + NADPH | native and recombinant enzymes, low activity | Sus scrofa | 1-phenylpropan-1-ol + NADP+ | - |
? | |
pyridine-3-aldehyde + NADPH | native and recombinant enzymes, low activity | Sus scrofa | pyridin-3-ylbutanol + NADP+ | - |
? | |
pyridine-4-aldehyde + NADPH + H+ | native and recombinant enzymes | Sus scrofa | pyridin-4-ylmethanol + NADP+ | - |
? | |
valerophenone + NADPH | native and recombinant enzymes | Sus scrofa | 1-phenylpentan-1-ol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | recombinant and native enzymes | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Sus scrofa |