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Literature summary for 1.1.1.184 extracted from

  • Shimada, H.; Fujiki, S.; Oginuma, M.; Asakawa, M.; Okawara, T.; Kato, K.; Yamamura, S.; Akita, H.; Hara, A.; Imamura, Y.
    Stereoselective reduction of 4-benzoylpyridine by recombinant pig heart carbonyl reductase (2003), J. Mol. Catal. B, 23, 29-35.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of the heart enzyme in Escherichia coli BL21(DE3), assembly to tetrameric form Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
hexaphenone pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.05
-
valerophenone pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.09
-
1,4-Naphthoquinone pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.09
-
pyridin-4-yl hexanoate pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.16
-
1,4-benzoquinone pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.17
-
n-butyrophenone pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.2
-
4-nitrobenzaldehyde pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.29
-
menadione pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.29
-
4-benzoylpyridine pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.76
-
3-benzoylpyridine pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.89
-
propiophenone pH 6.0, 25°C, recombinant enzyme Sus scrofa
0.93
-
2-benzoylpyridine pH 6.0, 25°C, recombinant enzyme Sus scrofa
1.2
-
4-Nitroacetophenone pH 6.0, 25°C, recombinant enzyme Sus scrofa
1.4
-
Pyridine-4-aldehyde pH 6.0, 25°C, recombinant enzyme Sus scrofa
11.4
-
Pyridine-3-aldehyde pH 6.0, 25°C, recombinant enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sus scrofa 5829
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-benzoquinone + NADPH native and recombinant enzymes, low activity Sus scrofa ? + NADP+
-
?
1,4-naphthoquinone + NADPH + H+ native and recombinant enzymes, low activity Sus scrofa ? + NADP+
-
?
2-benzoylpyridine + NADPH highly stereoselective reaction, native and recombinant enzymes, low activity Sus scrofa (S)-alpha-phenyl-2-pyridylmethanol + NADP+
-
?
3-benzoylpyridine + NADPH highly stereoselective reaction, native and recombinant enzymes Sus scrofa (S)-alpha-phenyl-3-pyridylmethanol + NADP+
-
?
4-benzoylpyridine + NADPH + H+ highly stereoselective reaction, native and recombinant enzymes Sus scrofa (S)-alpha-phenyl-4-pyridylmethanol + NADP+
-
?
4-hexanoylpyridine + NADPH + H+ native and recombinant enzymes Sus scrofa 1-(pyridin-4-yloxy)hexan-1-ol + NADP+
-
?
4-nitroacetophenone + NADPH native and recombinant enzymes, low activity Sus scrofa 1-(4-nitrophenyl)ethanol + NADP+
-
?
4-nitrobenzaldehyde + NADPH native and recombinant enzymes Sus scrofa 4-nitrobenzylalcohol + NADP+
-
?
hexaphenone + NADPH native and recombinant enzymes Sus scrofa 1-phenylhexan-1-ol + NADP+
-
?
menadione + NADPH native and recombinant enzymes, low activity Sus scrofa ? + NADP+
-
?
additional information substrate specificity, no activity with 3-nitroacetophenone and 4-acetylpyridine, catalytic activity of the recombinant enzyme is similar to the native enzyme Sus scrofa ?
-
?
n-butyrophenone + NADPH native and recombinant enzymes, low activity Sus scrofa 1-phenylbutan-1-ol + NADP+
-
?
propiophenone + NADPH native and recombinant enzymes, low activity Sus scrofa 1-phenylpropan-1-ol + NADP+
-
?
pyridine-3-aldehyde + NADPH native and recombinant enzymes, low activity Sus scrofa pyridin-3-ylbutanol + NADP+
-
?
pyridine-4-aldehyde + NADPH + H+ native and recombinant enzymes Sus scrofa pyridin-4-ylmethanol + NADP+
-
?
valerophenone + NADPH native and recombinant enzymes Sus scrofa 1-phenylpentan-1-ol + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer recombinant and native enzymes Sus scrofa

Synonyms

Synonyms Comment Organism
carbonyl reductase
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Sus scrofa