Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
genistein | 50% inhibition at 0.028 mM | Sus scrofa | |
kaempferol | 50% inhibition at 0.015 mM | Sus scrofa | |
myristic acid | 50% inhibition at 0.025 mM | Sus scrofa | |
quercetin | 50% inhibition at 0.016 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
9,10-phenanthrenequinone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.004 | - |
1-phenylisatin | pH 6.0, recombinant enzyme | Sus scrofa | |
0.021 | - |
hexanophenone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.028 | - |
heptanophenone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.03 | - |
isatin | pH 6.0, recombinant enzyme | Sus scrofa | |
0.031 | - |
nonanophenone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.04 | - |
1-phenyl-1,2-propanedione | pH 6.0, recombinant enzyme | Sus scrofa | |
0.052 | - |
valerophenone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.086 | - |
16-ketoestrone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.09 | - |
4-hexanoylpyridine | pH 6.0, recombinant enzyme | Sus scrofa | |
0.13 | - |
benzil | pH 6.0, recombinant enzyme | Sus scrofa | |
0.17 | - |
n-butyrophenone | pH 6.0, recombinant enzyme | Sus scrofa | |
0.29 | - |
menadione | pH 6.0, recombinant enzyme | Sus scrofa | |
0.29 | - |
4-benzoylpyridine | pH 6.0, recombinant enzyme | Sus scrofa | |
0.47 | - |
2,3-hexandione | pH 6.0, recombinant enzyme | Sus scrofa | |
0.76 | - |
3-benzoylpyridine | pH 6.0, recombinant enzyme | Sus scrofa | |
0.89 | - |
propiophenone | pH 6.0, recombinant enzyme | Sus scrofa | |
1.4 | - |
Pyridine-4-aldehyde | pH 6.0, recombinant enzyme | Sus scrofa | |
4.5 | - |
diacetyl | pH 6.0, recombinant enzyme | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, SDS-PAGE | Sus scrofa |
100000 | - |
about, gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | high expression level | Sus scrofa | - |
intestine | - |
Sus scrofa | - |
kidney | high expression level | Sus scrofa | - |
liver | - |
Sus scrofa | - |
additional information | tissue distribution, overview | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
purified recombinant enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenyl-1,2-propanedione + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
? | |
1-phenylisatin + NADPH | high activity, recombinant enzyme | Sus scrofa | ? + NADP+ | - |
? | |
16-ketoestrone + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
r | |
2,3-hexadione + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
? | |
3-benzoylpyridine + NADPH | recombinant enzyme | Sus scrofa | (S)-alpha-phenyl-3-pyridylmethanol + NADP+ | - |
? | |
4-benzoylpyridine + NADPH + H+ | recombinant enzyme | Sus scrofa | (S)-alpha-phenyl-4-pyridylmethanol + NADP+ | - |
? | |
4-hexanoylpyridine + NADPH + H+ | recombinant enzyme | Sus scrofa | 1-(pyridin-4-yloxy)hexan-1-ol + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | best substrate, recombinant enzyme | Sus scrofa | ? + NADP+ | - |
r | |
9-cis-retinal + NADPH | recombinant enzyme | Sus scrofa | 9-cis-retinol + NADP+ | - |
? | |
all-trans-retinal + NADPH + H+ | recombinant enzyme | Sus scrofa | all-trans-retinol + NADP+ | - |
? | |
benzil + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
? | |
diacetyl + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
? | |
heptanophenone + NADPH | recombinant enzyme | Sus scrofa | 1-phenylheptan-1-ol + NADP+ | - |
? | |
hexanophenone + NADPH | recombinant enzyme | Sus scrofa | 1-phenylhexan-1-ol + NADP+ | - |
? | |
isatin + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
r | |
menadione + NADPH | recombinant enzyme | Sus scrofa | ? + NADP+ | - |
? | |
additional information | substrate specificity, overview | Sus scrofa | ? | - |
? | |
n-butyrophenone + NADPH | recombinant enzyme | Sus scrofa | 1-phenylbutan-1-ol + NADP+ | - |
? | |
nonanophenone + NADPH | recombinant enzyme | Sus scrofa | 1-phenylnonan-1-ol + NADP+ | - |
? | |
propiophenone + NADPH | recombinant enzyme | Sus scrofa | 1-phenylpropan-1-ol + NADP+ | - |
? | |
pyridine-4-aldehyde + NADPH + H+ | recombinant enzyme | Sus scrofa | pyridin-4-ylmethanol + NADP+ | - |
? | |
valerophenone + NADPH | recombinant enzyme | Sus scrofa | 1-phenylpentan-1-ol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Sus scrofa |
CR | - |
Sus scrofa |
PHCR | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
about | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Sus scrofa |