Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by several chromatographic steps, i.e. Q-Sepharose, FPLC, Phenyl Sepharose, HiTrap Blue, Red Sepharose, and gel filtration, and native PAGE | Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(3,4,6-tri-[3,4,5,6-tetrahydro])phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-2-[(1,3-dioxo-1,3,4,5,6,7-hexahydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
2-(3,5-difluoro)phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-2-[(4,7-difluoro-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
2-(3-fluoro)phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-2-[(4-fluoro-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
2-(3-fluoro)phthalimido-methyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | methyl (3R)-2-[(4-fluoro-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
2-(3-methoxy)phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-3-hydroxy-2-[(4-methoxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]butanoate + NADP+ | - |
? | |
2-(4-fluoro)phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-2-[(5-fluoro-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
2-(4-methyl)phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-3-hydroxy-2-[(5-methyl-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]butanoate + NADP+ | - |
? | |
2-(4-methyl)phthalimido-methyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | methyl (3R)-3-hydroxy-2-[(5-methyl-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]butanoate + NADP+ | - |
? | |
2-(4-tert-butyl)phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | - |
Kluyveromyces marxianus | ethyl (3R)-2-[(5-tert-butyl-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
2-phthalimido-ethyl-3-oxobutanoate + NADPH + H+ | reduction of the beta-keto group to stereospecifically give the (R)-alcohol | Kluyveromyces marxianus | ethyl (3R)-2-[(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl]-3-hydroxybutanoate + NADP+ | - |
? | |
additional information | asymmetric reduction, anti- and syndiastereoselective reactions, overview, identification of products | Kluyveromyces marxianus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Kluyveromyces marxianus |