Application | Comment | Organism |
---|---|---|
synthesis | semienzymatic production of (R)-3-styrene oxide | Rhodotorula glutinis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenol | complete inhibition at 1 mM | Rhodotorula glutinis | |
Ag+ | complete inhibition at 1 mM | Rhodotorula glutinis | |
Diphenylhydantoin | 68% inhibition at 0.5 mM | Rhodotorula glutinis | |
DTNB | complete inhibition at 1 mM | Rhodotorula glutinis | |
Hg2+ | complete inhibition at 1 mM | Rhodotorula glutinis | |
iodoacetate | 19% inhibition at 1 mM | Rhodotorula glutinis | |
additional information | not affected by EDTA and 1,10-phenanthroline at 1 mM | Rhodotorula glutinis | |
NEM | 30% inhibition at 1 mM | Rhodotorula glutinis | |
p-chloromercuribenzoate | 76% inhibition at 1 mM | Rhodotorula glutinis | |
quercetin | complete inhibition at 0.1 mM | Rhodotorula glutinis | |
Zn2+ | 43% inhibition at 1 mM | Rhodotorula glutinis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 14% activation at 1 mM | Rhodotorula glutinis | |
Mn2+ | 20% activation at 1 mM | Rhodotorula glutinis | |
additional information | enzyme is not affected by Mg2+, Ca2+ and Cu2+ at 1 mM | Rhodotorula glutinis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, SDS-PAGE | Rhodotorula glutinis |
40000 | - |
gel filtration | Rhodotorula glutinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
var. dairenensis, IFO415 | - |
Purification (Comment) | Organism |
---|---|
487fold to homogeneity in a 4-step procedure | Rhodotorula glutinis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 8 | purified enzyme | Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromo-aceto-2-chlorophenone + NADPH | stereospecific enzyme | Rhodotorula glutinis | (R)-2-bromo-1-(2-chlorophenyl)ethanol + NADP+ | product reacts spontaneously to the (R)-styrene oxide derivative in NaOH | ? | |
2-bromo-aceto-3-chlorophenone + NADPH | stereospecific enzyme | Rhodotorula glutinis | (R)-2-bromo-1-(3-chlorophenyl)ethanol + NADP+ | product reacts spontaneously to the (R)-styrene oxide derivative in NaOH | ? | |
2-bromo-aceto-4-chlorophenone + NADPH | stereospecific enzyme | Rhodotorula glutinis | (R)-2-bromo-1-(4-chlorophenyl)ethanol + NADP+ | product reacts spontaneously to the (R)-styrene oxide derivative in NaOH | ? | |
2-bromo-acetophenone + NADPH | i.e. phenacyl bromide, stereospecific enzyme | Rhodotorula glutinis | (R)-2-bromo-1-phenylethanol + NADP+ | product reacts spontaneously to the (R)-styrene oxide derivative in NaOH | ? | |
2-butanone + NADPH | low activity | Rhodotorula glutinis | 2-butanol + NADP+ | - |
? | |
2-chloro-aceto-3-chlorophenone + NADPH | i.e. 3-chlorophenacyl chloride | Rhodotorula glutinis | (R)-2-chloro-1-(3-chlorophenyl)ethanol + NADP+ | product reacts spontaneously to the (R)-styrene oxide derivative in NaOH | ? | |
2-chlorobenzaldehyde + NADPH + H+ | - |
Rhodotorula glutinis | 2-chlorobenzylalcohol + NADP+ | - |
? | |
2-halo-acetophenone + NADPH | i.e. phenacyl halide, stereospecific enzyme | Rhodotorula glutinis | (R)-2-halo-1-phenylethanol + NADP+ | product reacts spontaneously to the (R)-styrene oxide derivative in NaOH | ? | |
2-heptanone + NADPH | - |
Rhodotorula glutinis | 2-heptanol + NADP+ | - |
? | |
2-nitrobenzaldehyde + NADPH | - |
Rhodotorula glutinis | 2-nitrobenzylalcohol + NADP+ | - |
? | |
2-oxobutyric acid + NADPH | low activity | Rhodotorula glutinis | 2-hydroxybutyric acid + NADP+ | - |
? | |
3-chlorobenzaldehyde + NADPH + H+ | - |
Rhodotorula glutinis | 3-chlorobenzylalcohol + NADP+ | - |
? | |
3-nitroacetophenone + NADPH | - |
Rhodotorula glutinis | 1-(3-nitrophenyl)ethanol + NADP+ | - |
? | |
3-nitrobenzaldehyde + NADPH | - |
Rhodotorula glutinis | 3-nitrobenzylalcohol + NADP+ | - |
? | |
4-acetylpyridine + NADPH | - |
Rhodotorula glutinis | ? + NADP+ | - |
? | |
4-chlorobenzaldehyde + NADPH + H+ | - |
Rhodotorula glutinis | 4-chlorobenzylalcohol + NADP+ | - |
? | |
4-nitrobenzaldehyde + NADPH | - |
Rhodotorula glutinis | 4-nitrobenzylalcohol + NADP+ | - |
? | |
acetone + NADPH | - |
Rhodotorula glutinis | 2-propanol + NADP+ | - |
? | |
benzaldehyde + NADPH | - |
Rhodotorula glutinis | benzylalcohol + NADP+ | - |
? | |
camphorquinone + NADPH | - |
Rhodotorula glutinis | ? + NADP+ | - |
? | |
cyclopentanone + NADPH | low activity | Rhodotorula glutinis | cyclopentanol + NADP+ | - |
? | |
diacetyl + NADPH | low activity | Rhodotorula glutinis | ? + NADP+ | - |
? | |
ethyl 2-chloro-3-oxobutanoate + NADPH + H+ | - |
Rhodotorula glutinis | ethyl 2-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Rhodotorula glutinis | ethyl 4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
glutaraldehyde + NADPH | low activity | Rhodotorula glutinis | ? + NADP+ | - |
? | |
ketopantoyl lactone + NADPH | - |
Rhodotorula glutinis | (R)-pantolactone + NADP+ | - |
? | |
methyl glyoxal + NADPH | - |
Rhodotorula glutinis | ? + NADP+ | - |
? | |
methyl pyruvate + NADPH | - |
Rhodotorula glutinis | methyl lactate + NADP+ | - |
? | |
additional information | enzyme is highly stereospecific, broad range substrate specificity | Rhodotorula glutinis | ? | - |
? | |
n-butylaldehyde + NADPH | - |
Rhodotorula glutinis | n-butanol + NADP+ | - |
? | |
n-hexylaldehyde + NADPH | low activity | Rhodotorula glutinis | n-hexanol + NADP+ | - |
? | |
octyl 4-chloro-3-oxobutanoate + NADPH | - |
Rhodotorula glutinis | octyl 4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
oxalacetic acid + NADPH | - |
Rhodotorula glutinis | ? + NADP+ | - |
? | |
propionaldehyde + NADPH | low activity | Rhodotorula glutinis | propanol + NADP+ | - |
? | |
propiophenone + NADPH | - |
Rhodotorula glutinis | 1-phenylpropan-1-ol + NADP+ | - |
? | |
pyridine-4-aldehyde + NADPH + H+ | - |
Rhodotorula glutinis | pyridin-4-ylmethanol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Rhodotorula glutinis |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase | - |
Rhodotorula glutinis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Rhodotorula glutinis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | assay at | Rhodotorula glutinis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Rhodotorula glutinis |