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Literature summary for 1.1.1.183 extracted from

  • Zhou, J.; Wang, C.; Yoon, S.; Jang, H.; Choi, E.; Kim, S.
    Engineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation (2014), J. Biotechnol., 169, 42-50.
    View publication on PubMed
Search for more literature for 1.1.1.183

Cloned(Commentary)

Cloned (Comment) Organism
gene GDH, functional recombinant expression in Escherichia coli strain MG-1655 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the overexpressing DHyjgB strain converts 33% of fed geraniol into other geranoids, which is much higher than the rate of the control strain with 7% Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + NADP+ Escherichia coli
-
 geranial + NADPH + H+
-
 r
additional information Escherichia coli geraniol, geranial, neral and nerol formations are sequentially observed during cell culture ?
-
 ?
nerol + NADP+ Escherichia coli
-
 neral + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli P27250
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + NADP+
-
 Escherichia coli geranial + NADPH + H+
-
 r
additional information geraniol, geranial, neral and nerol formations are sequentially observed during cell culture Escherichia coli ?
-
 ?
nerol + NADP+
-
 Escherichia coli neral + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
geraniol dehydrogenase-like enzyme
-
 Escherichia coli
More cf. EC 1.1.1.2 Escherichia coli
YjgB
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli
NADPH
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction in the enzyme-deficient MGDELTAyjgB strain, conversion of geraniol to other geranoids is significantly reduced to 11%. Similar patterns are observed when feeding nerol and citral. In the MGDELTAyjgB strain, conversion of fed nerol to other geranoids is reduced from 12% to 4%, and conversion of neral and geranial to other geranoids during the citral feeding experiment is also reduced by almost 50% in both cases Escherichia coli
metabolism YjgB is the major enzyme responsible for the endogenous formation of geranoids during geraniol production in Escherichia coli Escherichia coli