KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
scyllo-inosose | - |
Galdieria sulphuraria | |
430 | - |
myo-inositol | - |
Galdieria sulphuraria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Galdieria sulphuraria |
160000 | - |
density gradient centrifugation | Galdieria sulphuraria |
180000 | - |
gel filtration | Galdieria sulphuraria |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Galdieria sulphuraria | 75 times lower activity compared to the reverse reaction | scyllo-inosose + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Galdieria sulphuraria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Galdieria sulphuraria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Galdieria sulphuraria | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Galdieria sulphuraria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epi-inositol + NAD+ | 5% of the activity compared to myo-inositol as substrate, conversion of epi-inosose 5% of the activity compared to scyllo-inosose as substrate | Galdieria sulphuraria | epi-inosose + NADH | - |
r | |
myo-inositol + NAD+ | 75 times lower activity compared to the reverse reaction | Galdieria sulphuraria | scyllo-inosose + NADH | - |
r | |
scyllo-inositol + NAD+ | 5% of the activity compared to myo-inositol as substrate | Galdieria sulphuraria | scyllo-inosose + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Galdieria sulphuraria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for 2 h | Galdieria sulphuraria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
clearly favours reduction of scyllo-inosose | Galdieria sulphuraria |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | half-maximal activity at pH 5.5 and pH 9.5 | Galdieria sulphuraria |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Galdieria sulphuraria | |
NADH | - |
Galdieria sulphuraria |