Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Paenarthrobacter nicotinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
NADP+ | 28°C, pH 7.8 | Paenarthrobacter nicotinovorans | |
5.03 | - |
NAD+ | 28°C, pH 7.8 | Paenarthrobacter nicotinovorans | |
15.2 | - |
D-xylose | 28°C, pH 7.8, with NADP+ as cofactor | Paenarthrobacter nicotinovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 2 mol Zn2+ per monomer | Paenarthrobacter nicotinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
4 * 47000, SDS-PAGE | Paenarthrobacter nicotinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | very low activity compared to NADP+ | Paenarthrobacter nicotinovorans | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
D-xylose + NADP+ | - |
Paenarthrobacter nicotinovorans | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | at concentrations of up to 50 mM of L-xylose, D-ribose, D-arabinose, L-arabinose, D-glucose, D-galactose or D-fructose, the enzyme produces little NADPH and the specific activity toward these substrates are below 5% of the activity recorded with D-xylose | Paenarthrobacter nicotinovorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 47000, SDS-PAGE | Paenarthrobacter nicotinovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
NAD+ | 28°C, pH 7.8 | Paenarthrobacter nicotinovorans | |
10.5 | - |
NADP+ | 28°C, pH 7.8 | Paenarthrobacter nicotinovorans | |
14.9 | - |
D-xylose | 28°C, pH 7.8, with NADP+ as cofactor | Paenarthrobacter nicotinovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | very low activity compared to NADP+ | Paenarthrobacter nicotinovorans | |
NADP+ | strong preference for NADP+ | Paenarthrobacter nicotinovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.298 | - |
NAD+ | 28°C, pH 7.8 | Paenarthrobacter nicotinovorans | |
0.98 | - |
D-xylose | 28°C, pH 7.8, with NADP+ as cofactor | Paenarthrobacter nicotinovorans | |
32 | - |
NADP+ | 28°C, pH 7.8 | Paenarthrobacter nicotinovorans |