Cloned (Comment) | Organism |
---|---|
wild-type protein and mutants are expressed in Escherichia coli BL21 (DE3) | Macaca fascicularis |
Crystallization (Comment) | Organism |
---|---|
crystals are obtained at 19.9°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric D-xylose dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution | Macaca fascicularis |
Protein Variants | Comment | Organism |
---|---|---|
A36D | site-directed mutagenesis | Macaca fascicularis |
D280A | by site-directed mutagenesis | Macaca fascicularis |
F154A | by site-directed mutagenesis | Macaca fascicularis |
F279A | by site-directed mutagenesis | Macaca fascicularis |
H76Q | by site-directed mutagenesis | Macaca fascicularis |
H79Q | by site-directed mutagenesis | Macaca fascicularis |
K97M | by site-directed mutagenesis | Macaca fascicularis |
K97R | by site-directed mutagenesis | Macaca fascicularis |
R37A | site-directed mutagenesis | Macaca fascicularis |
R37D | site-directed mutagenesis | Macaca fascicularis |
R41A | by site-directed mutagenesis | Macaca fascicularis |
R41D | by site-directed mutagenesis | Macaca fascicularis |
W125Y | by site-directed mutagenesis | Macaca fascicularis |
W254A | by site-directed mutagenesis | Macaca fascicularis |
W254Y | by site-directed mutagenesis | Macaca fascicularis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxyacetophenone | - |
Macaca fascicularis | |
isoascorbic acid | competitive inhibitor | Macaca fascicularis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
D-xylose | oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
2.9 | - |
D-xylose | oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
3.4 | - |
D-xylose | oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
4.4 | - |
D-xylose | oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
8 | 9 | D-xylose | oxidation reaction of mutant F279A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
11 | - |
D-xylose | oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
14 | - |
D-xylose | oxidation reaction of mutant W254Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
15 | - |
D-xylose | oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
36 | - |
D-xylose | oxidation reaction of mutant F154A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
41 | - |
D-xylose | oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
65 | - |
D-xylose | oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
67 | - |
D-xylose | oxidation reaction of mutant W125Y, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis | |
90 | - |
D-xylose | oxidation reaction of mutant W254A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM d-xylose | Macaca fascicularis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADP+ | Macaca fascicularis | enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | Macaca fascicularis | D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca fascicularis | Q9TQS6 | - |
- |
Purification (Comment) | Organism |
---|---|
the recombinant enzymes are purified from the 12000g supernatants of the homogenates of the cells | Macaca fascicularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADP+ | enzyme has dehydrogenase and reductase activities, enzyme is identical to dihydrodiol dehydrogenase | Macaca fascicularis | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | D-xylose dehydrogenase catalyzes the NADP+-linked oxidation of dihydrodiols of aromatic hydrocarbons to their corresponding catechols and is regarded as a toxication enzyme in the metabolism of carcinogenic polycyclic aromatic hydrocarbons because oxidation by the enzyme yields reactive and redox-active ortho-quinones and reactive oxygen species, broad substrate specificity indicates additional roles of the enzyme in the metabolism of endogenous and xenobiotic carbonyl compounds and the prevention of the development of glycation | Macaca fascicularis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Macaca fascicularis |
Synonyms | Comment | Organism |
---|---|---|
NADP+-dependent D-xylose dehydrogenase | - |
Macaca fascicularis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
D-xylose | oxidation reaction of mutant R37D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
0.45 | - |
D-xylose | oxidation reaction of mutant A36D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
0.5 | - |
D-xylose | oxidation reaction of mutant H76Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
0.9 | - |
D-xylose | oxidation reaction of mutant H79Q, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
1 | - |
D-xylose | oxidation reaction of mutant R41D, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
1.2 | - |
D-xylose | oxidation reaction of mutant R37A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
1.7 | - |
D-xylose | oxidation reaction of mutant R41A, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis | |
2.6 | - |
D-xylose | oxidation reaction of wild type enzyme, 25 mM Tris-HCl pH 7.5, 0.25 mM NADP+, 50 mM D-xylose | Macaca fascicularis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Macaca fascicularis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Macaca fascicularis |