KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
2-methyl-3-hydroxybutyryl-CoA | - |
Pseudomonas putida | |
0.5 | - |
3-hydroxybutyryl-CoA | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration, sucrose density gradient centrifugation | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-hydroxybutyryl-CoA + NAD+ | Pseudomonas putida | isoleucine metabolism | 2-methylacetoacetyl-CoA + NADH | - |
? | |
2-methyl-3-hydroxybutyryl-CoA + NAD+ | Pseudomonas putida PpG2 | isoleucine metabolism | 2-methylacetoacetyl-CoA + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
PpG2 | - |
Pseudomonas putida PpG2 | - |
PpG2 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH + H+ | inducible by tiglate | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.2 | - |
2-methyl-3-hydroxybutyryl-CoA | Pseudomonas putida |
21.7 | - |
3-hydroxybutyryl-CoA | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-3-methylpentanoyl-CoA + NAD+ | - |
Pseudomonas putida | 3-methyl-2-oxopentanoyl-CoA + NADH | - |
? | |
2-hydroxy-3-methylpentanoyl-CoA + NAD+ | - |
Pseudomonas putida PpG2 | 3-methyl-2-oxopentanoyl-CoA + NADH | - |
? | |
2-methyl-3-hydroxybutyryl-CoA + NAD+ | - |
Pseudomonas putida | 2-methylacetoacetyl-CoA + NADH | - |
? | |
2-methyl-3-hydroxybutyryl-CoA + NAD+ | isoleucine metabolism | Pseudomonas putida | 2-methylacetoacetyl-CoA + NADH | - |
? | |
2-methyl-3-hydroxybutyryl-CoA + NAD+ | - |
Pseudomonas putida PpG2 | 2-methylacetoacetyl-CoA + NADH | - |
? | |
2-methyl-3-hydroxybutyryl-CoA + NAD+ | isoleucine metabolism | Pseudomonas putida PpG2 | 2-methylacetoacetyl-CoA + NADH | - |
? | |
3-hydroxybutyryl-CoA + NAD+ | - |
Pseudomonas putida | acetoacetyl-CoA + NADH | - |
? | |
3-hydroxybutyryl-CoA + NAD+ | - |
Pseudomonas putida PpG2 | acetoacetyl-CoA + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cannot be replaced by NADP+ | Pseudomonas putida |