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Literature summary for 1.1.1.176 extracted from

  • Eggert, T.; Bakonyi, D.; Hummel, W.
    Enzymatic routes for the synthesis of ursodeoxycholic acid (2014), J. Biotechnol., 191, 11-21.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Clostridium sp.
synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Clostridium perfringens
synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases Eggerthella lenta
synthesis the enzyme is useful in production of ursodeoxycholic acid, a secondary bile acid, which is used as a drug for the treatment of various liver diseases [Clostridium] leptum

Protein Variants

Protein Variants Comment Organism
additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Clostridium perfringens
additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Eggerthella lenta
additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview [Clostridium] leptum
additional information development and evaluation of a selected multi-step reaction system for the synthesis of ursodeoxycholic acid. Multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum, method, separation of each step by isolation of the intermediates using ultrafiltration membranes, overview Clostridium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholate + NAD+ Clostridium perfringens
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
?
cholate + NAD+ Eggerthella lenta
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
?
cholate + NADP+ Clostridium sp.
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ [Clostridium] leptum
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ Clostridium sp. ATCC 29733
-
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
additional information Clostridium sp. multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum ?
-
?
additional information Clostridium sp. ATCC 29733 multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-
Clostridium sp.
-
group P C 48-50
-
Clostridium sp. ATCC 29733
-
group P C 48-50
-
Eggerthella lenta
-
formerly Eubacterium lentum
-
[Clostridium] leptum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholate + NAD+
-
Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
?
cholate + NAD+
-
Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
?
cholate + NADP+
-
Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+
-
[Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium sp. 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium perfringens 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+
-
Clostridium sp. ATCC 29733 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
cholate + NADP+ conversion of CA into 12-oxoursodeoxycholic acid Clostridium sp. ATCC 29733 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
?
additional information multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum Clostridium sp. ?
-
?
additional information multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum Clostridium sp. ATCC 29733 ?
-
?

Synonyms

Synonyms Comment Organism
12alpha-HSDH
-
Clostridium sp.
12alpha-HSDH
-
Clostridium perfringens
12alpha-HSDH
-
Eggerthella lenta
12alpha-HSDH
-
[Clostridium] leptum
NAD+-dependent 12alpha-HSDH
-
Clostridium perfringens
NAD+-dependent 12alpha-HSDH
-
Eggerthella lenta
NADP+-dependent 12alpha-HSDH
-
Clostridium sp.
NADP+-dependent 12alpha-HSDH
-
[Clostridium] leptum

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+-dependent 12alpha-HSDH Clostridium perfringens
NAD+ NAD+-dependent 12alpha-HSDH Eggerthella lenta
NADP+ NADP+-dependent 12alpha-HSDH Clostridium sp.
NADP+ NADP+-dependent 12alpha-HSDH [Clostridium] leptum