KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
3alpha,12alpha-dihydroxy-5beta-cholanoyl taurine | - |
[Clostridium] leptum | |
0.57 | - |
3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine | - |
[Clostridium] leptum | |
1.6 | - |
3alpha,12alpha-dihydroxy-5beta-cholanoate | - |
[Clostridium] leptum | |
2.7 | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine | - |
[Clostridium] leptum | |
4 | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine | - |
[Clostridium] leptum | |
7 | - |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoate | - |
[Clostridium] leptum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
225000 | - |
gel filtration | [Clostridium] leptum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] leptum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Clostridium] leptum |
Storage Stability | Organism |
---|---|
-20°C, 20 days, 50% loss of activity | [Clostridium] leptum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholic acid + NAD(P)+ | - |
[Clostridium] leptum | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
deoxycholic acid + NAD(P)+ | i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid | [Clostridium] leptum | 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ | - |
? | |
glycocholic acid + NAD+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine | [Clostridium] leptum | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
glycodeoxycholic acid + NAD+ | i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine | [Clostridium] leptum | 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
taurocholic acid + NAD(P)+ | - |
[Clostridium] leptum | 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H | - |
? | |
taurodeoxycholic acid + NAD+ | - |
[Clostridium] leptum | 3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
4 | half-life: 11 days | [Clostridium] leptum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | oxidation of cholic acid | [Clostridium] leptum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
[Clostridium] leptum |