Cloned (Comment) | Organism |
---|---|
gene xylB, recombinnat expression in Pichia kudriavzevii strain VTT C-79090T | Caulobacter vibrioides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of non-conventional yeast Pichia kudriavzevii strain VTT C-79090T to recombinantly express the D-xylose dehydrogenase coding gene from Caulobacter crescentus. With this single modification the recombinant strain VTT C-79090T produces up to 171 g/l of D-xylonate from 171 g/l D-xylose at a rate of 1.4 g/l/h and a high yield, which is comparable with D-xylonate production by Gluconobacter oxydans or Pseudomonas sp. The productivity of the strain is also remarkably high at low pH, producing 146 g/l D-xylonate at pH 3.0. Comparison to production of D-xylonate from Saccharomyces cerevisiae strain VTT B-67002 expressing the gene from Caulobacter crescentus, overview | Caulobacter vibrioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | Caulobacter vibrioides | - |
D-xylonolactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | Caulobacter vibrioides NA1000 | - |
D-xylonolactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | B8H1Z0 | - |
- |
Caulobacter vibrioides NA1000 | B8H1Z0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | - |
Caulobacter vibrioides | D-xylonolactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | - |
Caulobacter vibrioides NA1000 | D-xylonolactone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-xylose 1-dehydrogenase | - |
Caulobacter vibrioides |
XylB | - |
Caulobacter vibrioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Caulobacter vibrioides |