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Literature summary for 1.1.1.17 extracted from

  • Bonin, P.; Groisillier, A.; Raimbault, A.; Guibert, A.; Boyen, C.; Tonon, T.
    Molecular and biochemical characterization of mannitol-1-phosphate dehydrogenase from the model brown alga Ectocarpus sp. (2015), Phytochemistry, 117, 509-520.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene MPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis Ectocarpus siliculosus
gene MPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis, recombinant expression of His-tagged EsM1PDH1 in Escherichia coli Ectocarpus siliculosus
gene MPDH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis Ectocarpus siliculosus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA; no inhibition by EDTA; no inhibition by EDTA Ectocarpus siliculosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Ectocarpus siliculosus
0.02
-
NAD+ recombinant enzyme, pH 9.0, 30°C Ectocarpus siliculosus
0.19
-
D-fructose 6-phosphate recombinant enzyme, pH 7.0, 30°C Ectocarpus siliculosus
0.38
-
D-mannitol 1-phosphate recombinant enzyme, pH 9.0, 30°C Ectocarpus siliculosus
16
-
NADH recombinant enzyme, pH 7.0, 30°C Ectocarpus siliculosus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activates Ectocarpus siliculosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
Ectocarpus siliculosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol 1-phosphate + NAD+ Ectocarpus siliculosus
-
D-fructose 6-phosphate + NADH + H+
-
r
additional information Ectocarpus siliculosus mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22) ?
-
?

Organism

Organism UniProt Comment Textmining
Ectocarpus siliculosus D7FMJ8 MPDH1
-
Ectocarpus siliculosus D7G793 MPDH3
-
Ectocarpus siliculosus D8LI09 MPDH2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged EsM1PDH1 from Escherichia coli by nickel affinity chromatography and gel filtration Ectocarpus siliculosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
recombinant enzyme, D-mannitol 1-phosphate oxidation, pH 9.0, 30°C Ectocarpus siliculosus
0.016
-
purified recombinant enzyme, D-mannitol 1-phosphate oxidation, pH 9.0, 30°C Ectocarpus siliculosus
0.026
-
recombinant enzyme, D-fructose 6-phosphate reduction, pH 7.0, 30°C Ectocarpus siliculosus
0.046
-
purified recombinant enzyme, D-fructose 6-phosphate reduction, pH 7.0, 30°C Ectocarpus siliculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol 1-phosphate + NAD+
-
Ectocarpus siliculosus D-fructose 6-phosphate + NADH + H+
-
r
additional information mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22) Ectocarpus siliculosus ?
-
?
additional information EsM1PDH1cat isozyme is specific for D-fructose 6-phosphate and D-mannitol 1-phosphate Ectocarpus siliculosus ?
-
?

Subunits

Subunits Comment Organism
? x * 42000, recombinant His-tagged EsM1PDH1 isozyme, SDS-PAGE Ectocarpus siliculosus
More structure-based sequence comparisons, overview Ectocarpus siliculosus

Synonyms

Synonyms Comment Organism
EsM1PDH1
-
Ectocarpus siliculosus
EsM1PDH2
-
Ectocarpus siliculosus
EsM1PDH3
-
Ectocarpus siliculosus
M1PDH
-
Ectocarpus siliculosus
mannitol-1-phosphate dehydrogenase
-
Ectocarpus siliculosus
MPDH1
-
Ectocarpus siliculosus
MPDH2
-
Ectocarpus siliculosus
MPDH3
-
Ectocarpus siliculosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Ectocarpus siliculosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 50 35% of maximum activity at 5-10°C, less than 5% at 50°C Ectocarpus siliculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
D-fructose 6-phosphate reduction Ectocarpus siliculosus
9
-
D-mannitol 1-phosphate oxidation Ectocarpus siliculosus

Cofactor

Cofactor Comment Organism Structure
additional information isozyme EsM1PDH1 highly prefers NADH/NAD+ to NADP+/NADPH Ectocarpus siliculosus
NAD+
-
Ectocarpus siliculosus
NADH
-
Ectocarpus siliculosus

General Information

General Information Comment Organism
evolution sequence analysis suggests that algal and apicomplexa M1PDHs represent a distinct type of mannitol-1-phosphate dehydrogenase in the PSLDR family Ectocarpus siliculosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29
-
D-mannitol 1-phosphate recombinant enzyme, pH 9.0, 30°C Ectocarpus siliculosus
170
-
D-fructose 6-phosphate recombinant enzyme, pH 7.0, 30°C Ectocarpus siliculosus