Cloned (Comment) | Organism |
---|---|
gene BbMPD, DNA and amino acid sequence determination and analysis, phylogenetic tree | Beauveria bassiana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 35.9% inhibition at 10 mM of the reverse reaction | Beauveria bassiana | |
Cu2+ | 32.8% inhibition at 2 mM of the reverse reaction | Beauveria bassiana | |
DTT | 46.0% inhibition at 10 mM of the reverse reaction | Beauveria bassiana | |
Li+ | 24.4% inhibition at 50 mM of the reverse reaction | Beauveria bassiana | |
Mg2+ | 31.2% inhibition at 10 mM of the reverse reaction | Beauveria bassiana | |
Mn2+ | 31.3% inhibition at 50 mM of the reverse reaction | Beauveria bassiana | |
additional information | no or poor inhibition of the reverse reaction by EDTA and 2-mercaptoethanol | Beauveria bassiana | |
SDS | 80.1% inhibition at 1 mM of the reverse reaction | Beauveria bassiana | |
Zn2+ | 44.7% inhibition at 2 mM of the reverse reaction | Beauveria bassiana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | Beauveria bassiana | - |
D-fructose 6-phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beauveria bassiana | C7FDI4 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme | Beauveria bassiana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
673.7 | - |
pH 7.0, 37°C, purified enzyme | Beauveria bassiana |
Storage Stability | Organism |
---|---|
4°C, purified native enzyme, the enzyme retains high activity during storage for two weeks | Beauveria bassiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | - |
Beauveria bassiana | D-fructose 6-phosphate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BbMPD | - |
Beauveria bassiana |
long-chain mannitol-1-phosphate dehydrogenase | - |
Beauveria bassiana |
mannitol-1-phosphate dehydrogenase | - |
Beauveria bassiana |
MPD | - |
Beauveria bassiana |
NAD+-dependent mannitol-1-phosphate dehydrogenase | - |
Beauveria bassiana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Beauveria bassiana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | activity range, profile, overview | Beauveria bassiana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Beauveria bassiana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | activity range, profile, overview | Beauveria bassiana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Beauveria bassiana | |
NADH | - |
Beauveria bassiana |
Organism | Comment | Expression |
---|---|---|
Beauveria bassiana | induction of the enzyme in cell culture by isopropyl 1-thio-beta-D-galactopyranoside | up |
General Information | Comment | Organism |
---|---|---|
physiological function | crucial role of BbMPD in the mannitol biosynthesis of Beauveria bassiana | Beauveria bassiana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1310 | - |
D-fructose 6-phosphate | pH 7.0, 37°C | Beauveria bassiana |