Crystallization (Comment) | Organism |
---|---|
fine needle, at about 40% ammonium sulfate saturation | Stenotrophomonas maltophilia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Barbital | 9.0% at 1 mM | Stenotrophomonas maltophilia | |
D-pantoic acid | noncompetitive with ketopantoic acid at saturation level of NADPH, noncompetitive with NADPH at unsaturation level of ketopantoic acid | Stenotrophomonas maltophilia | |
Diphenylhydantoin | 22.6% at 1 mM | Stenotrophomonas maltophilia | |
iodoacetate | sulfhydryl reagents, slightly inhibitory | Stenotrophomonas maltophilia | |
NaAsO2 | carbonyl reagent | Stenotrophomonas maltophilia | |
NADP+ | competitive with NADPH at unsaturation level of ketopantoic acid | Stenotrophomonas maltophilia | |
p-chloromercuribenzoate | sulfhydryl reagents, slightly inhibitory | Stenotrophomonas maltophilia | |
phenylhydrazine | carbonyl reagent | Stenotrophomonas maltophilia | |
quercetin | 12.0% at 0.1 mM | Stenotrophomonas maltophilia | |
Semicarbazide | carbonyl reagent | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0318 | - |
NADPH | pH 7.0 | Stenotrophomonas maltophilia | |
0.4 | - |
ketopantoic acid | pH 7.0 | Stenotrophomonas maltophilia | |
8.55 | - |
2-Keto-3-hydroxyisovalerate | - |
Stenotrophomonas maltophilia | |
52.1 | - |
D-pantoic acid | pH 8.0 | Stenotrophomonas maltophilia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ independent | Stenotrophomonas maltophilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
3-5 * 30500 or 30000, SDS-PAGE | Stenotrophomonas maltophilia |
87000 | - |
+/-5000, sedimentation equilibrium method | Stenotrophomonas maltophilia |
115000 | - |
+/-5000, gel filtration | Stenotrophomonas maltophilia |
140000 | 160000 | +/-5000, HPLC gel filtration | Stenotrophomonas maltophilia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Stenotrophomonas maltophilia | - |
845 | - |
Stenotrophomonas maltophilia 845 | - |
845 | - |
Purification (Comment) | Organism |
---|---|
gel chromatography | Stenotrophomonas maltophilia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
633 | - |
crystalline enzyme | Stenotrophomonas maltophilia |
669 | - |
- |
Stenotrophomonas maltophilia |
Storage Stability | Organism |
---|---|
4°C, 0.01 M Tris/HCl buffer, pH 7.4, 0.1 mM DTT, 0.2 M NaCl, 6 months | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-keto-3-hydroxyisovalerate + NADPH | - |
Stenotrophomonas maltophilia | ? + NADP+ | - |
r | |
2-keto-3-hydroxyisovalerate + NADPH | - |
Stenotrophomonas maltophilia 845 | ? + NADP+ | - |
r | |
ketopantoic acid + NADPH | - |
Stenotrophomonas maltophilia | D-pantoic acid + NADP+ | - |
r | |
ketopantoic acid + NADPH | - |
Stenotrophomonas maltophilia 845 | D-pantoic acid + NADP+ | - |
r | |
additional information | high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate | Stenotrophomonas maltophilia | ? | - |
? | |
additional information | high specificity, among a variety of carbonyl compounds only ketopantoic acid and 2-keto-3-hydroxyisovalerate can serve as substrate | Stenotrophomonas maltophilia 845 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | 3-5 * 30500 or 30000, SDS-PAGE | Stenotrophomonas maltophilia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
reduction of ketopantoic acid | Stenotrophomonas maltophilia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | 15°C: about 70% of activity maximum, 60°C: about 85% of activity maximum | Stenotrophomonas maltophilia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, pH 6-10 | Stenotrophomonas maltophilia |
60 | - |
10 min, stable below 60°C | Stenotrophomonas maltophilia |
70 | - |
10 min, 70% loss of activity | Stenotrophomonas maltophilia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of ketopantoic acid | Stenotrophomonas maltophilia |
8.5 | - |
oxidation of D-pantoic acid | Stenotrophomonas maltophilia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 30°C, 30 min | Stenotrophomonas maltophilia |
7 | - |
stable below 60°C for 10 min | Stenotrophomonas maltophilia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Stenotrophomonas maltophilia | |
NADP+ | - |
Stenotrophomonas maltophilia | |
NADPH | - |
Saccharomyces cerevisiae | |
NADPH | specific | Stenotrophomonas maltophilia |