Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.165 extracted from

  • Miyoshi, T.; Sato, H.; Harada, T.
    Purification and characterization of 2-alkyne-1-ol dehydrogenase induced by 2-butyne-1,4-diol in Fusarium merismoides B11 (1974), Biochim. Biophys. Acta, 358, 231-239.
No PubMed abstract available
Search for more literature for 1.1.1.165

General Stability

General Stability Organism
highly labile, even in the presence of DTT Fusarium merismoides

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 complete inhibition at 2 mM Fusarium merismoides
HgCl2 complete inhibition at 2 mM Fusarium merismoides
iodoacetic acid complete inhibition at 2 mM Fusarium merismoides
KCN slight inhibition at 2 mM Fusarium merismoides
NaN3 slight inhibition at 2 mM Fusarium merismoides
Ni2+ 84% inhibition at 2 mM Fusarium merismoides
p-chloromercuribenzoate complete inhibition at 2 mM Fusarium merismoides
Zn2+ 55% inhibition at 2 mM Fusarium merismoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.1
-
 2-butyne-1,4-diol
-
 Fusarium merismoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-butyne-1,4-diol + NAD+ Fusarium merismoides
-
 4-hydroxybut-2-ynal + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium merismoides
-
 strainB11
-
Fusarium merismoides B11
-
 strainB11
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Fusarium merismoides

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:2-butyne-1,4-diol-grown cell highest induction of enzyme formation by use of 2-butyne-1,4-diol-containing growth media Fusarium merismoides
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
751.1
-
-
 Fusarium merismoides

Storage Stability

Storage Stability Organism
5°C, 2 days Fusarium merismoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,4-butanetriol + NAD+ low activity Fusarium merismoides ? + NADH
-
 ?
1,2,4-butanetriol + NAD+ low activity Fusarium merismoides B11 ? + NADH
-
 ?
1,2-butanediol + NAD+ low activity Fusarium merismoides 1-hydroxybutan-2-one + NADH
-
 ?
1,2-butanediol + NAD+ low activity Fusarium merismoides B11 1-hydroxybutan-2-one + NADH
-
 ?
1,2-propanediol + NAD+
-
 Fusarium merismoides 1-hydroxy-2-propanal + NADH
-
 ?
1,2-propanediol + NAD+
-
 Fusarium merismoides B11 1-hydroxy-2-propanal + NADH
-
 ?
1,4-butanediol + NAD+
-
 Fusarium merismoides 1-hydroxy-4-butanal + NADH
-
 ?
1,4-butanediol + NAD+
-
 Fusarium merismoides B11 1-hydroxy-4-butanal + NADH
-
 ?
1,6-hexanediol + NAD+ low activity Fusarium merismoides 1-hydroxy-6-hexanal + NADH
-
 ?
1-butene-3-ol + NAD+ low activity Fusarium merismoides 1-buten-3-one + NADH
-
 ?
1-butyne-3-ol + NAD+ very low activity Fusarium merismoides 1-butyne-3-one + NADH
-
 ?
2-butene-1-ol + NAD+ cis-trans mixture, very low activity Fusarium merismoides 2-butene-1-al + NADH
-
 ?
2-butyne-1,4-diol + NAD+
-
 Fusarium merismoides 4-hydroxybut-2-ynal + NADH
-
 ?
2-butyne-1-ol + NAD+ highest activity Fusarium merismoides 2-butyn-1-al + NADH
-
 ?
2-propene-1-ol + NAD+ low activity Fusarium merismoides 2-propene-1-al + NADH
-
 ?
2-propyne-1-ol + NAD+
-
 Fusarium merismoides 2-propyn-1-al + NADH
-
 ?
3-butyne-1-ol + NAD+ very low activity Fusarium merismoides 3-butyn-1-al + NADH
-
 ?
n-propanol + NAD+ low activity Fusarium merismoides n-propanal + NADH
-
 ?
n-propanol + NAD+ low activity Fusarium merismoides B11 n-propanal + NADH
-
 ?
trans-2-butene-1,4-diol + NAD+
-
 Fusarium merismoides ? + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below, rapid decrease of activity above Fusarium merismoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Fusarium merismoides

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 8.5 stable between Fusarium merismoides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Fusarium merismoides
NADP+ one third of the activity compared to NAD+ Fusarium merismoides