KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
Phenanthrenequinone | - |
Gallus gallus | |
0.11 | - |
D-erythrulose | - |
Gallus gallus | |
0.19 | - |
3,4-hexanedione | - |
Gallus gallus | |
0.24 | - |
2,3-Pentanedione | - |
Gallus gallus | |
0.26 | - |
2,3-heptanedione | - |
Gallus gallus | |
0.39 | - |
diacetyl | - |
Gallus gallus | |
0.67 | - |
1,2-Cyclohexanedione | - |
Gallus gallus | |
0.68 | - |
(1S)-(+)-Camphorquinone | - |
Gallus gallus | |
0.72 | - |
isatin | - |
Gallus gallus | |
1.1 | - |
ethyl pyruvate | - |
Gallus gallus | |
1.1 | - |
Methyl pyruvate | - |
Gallus gallus | |
13 | - |
acetoin | - |
Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NAD(P)H | Gallus gallus | reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5 | acetoin + NADP+ | - |
ir | |
diacetyl + NAD(P)H | Gallus gallus | detoxification of food derived dicarbonyls | acetoin + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Gallus gallus | - |
brain | - |
Gallus gallus | - |
heart | - |
Gallus gallus | - |
intestine | - |
Gallus gallus | - |
kidney | - |
Gallus gallus | - |
liver | - |
Gallus gallus | - |
additional information | minimal activity also in lens, spleen, lung and pancreas | Gallus gallus | - |
stomach | - |
Gallus gallus | - |
testis | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
acetoin | Gallus gallus |
64 | - |
(1S)-(+)-camphorquinone | Gallus gallus |
77 | - |
methyl pyruvate | Gallus gallus |
83 | - |
phenanthrenequione | Gallus gallus |
120 | - |
2,3-pentanedione | Gallus gallus |
120 | - |
ethyl pyruvate | Gallus gallus |
120 | - |
1,2-cyclohexanedione | Gallus gallus |
130 | - |
diacetyl | Gallus gallus |
180 | - |
2,3-heptanedione | Gallus gallus |
200 | - |
3,4-hexanedione | Gallus gallus |
250 | - |
isatin | Gallus gallus |
320 | - |
purified enzyme, D-erythrulose | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1S)-(+)-camphorquinone + NADPH | stereospecific | Gallus gallus | ? | - |
? | |
1,2-cyclohexanedione + NADPH | - |
Gallus gallus | ? | - |
? | |
2,3-heptanedione + NADPH | - |
Gallus gallus | ? | - |
? | |
2,3-pentanedione + NADPH | - |
Gallus gallus | ? | - |
? | |
3,4-hexanedione + NADPH | - |
Gallus gallus | ? | - |
? | |
acetoin + NADPH + H+ | only slight reversibility, low activity | Gallus gallus | 2,3-butanediol + NADP+ | - |
r | |
D-erythrulose + NAD(P)H | - |
Gallus gallus | D-threitol + NAD(P)+ | - |
r | |
diacetyl + NAD(P)H | utilizes mainly vicinal alpha-dicarbonyls | Gallus gallus | acetoin + NADP+ | - |
? | |
diacetyl + NAD(P)H | utilizes mainly vicinal alpha-dicarbonyls | Gallus gallus | acetoin + NADP+ | - |
ir | |
diacetyl + NAD(P)H | reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5 | Gallus gallus | acetoin + NADP+ | - |
? | |
diacetyl + NAD(P)H | reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5 | Gallus gallus | acetoin + NADP+ | - |
ir | |
diacetyl + NAD(P)H | specific for alpha-dicarbonyls | Gallus gallus | acetoin + NADP+ | - |
? | |
diacetyl + NAD(P)H | specific for alpha-dicarbonyls | Gallus gallus | acetoin + NADP+ | - |
ir | |
diacetyl + NAD(P)H | detoxification of food derived dicarbonyls | Gallus gallus | acetoin + NADP+ | - |
ir | |
ethyl pyruvate + NADPH | - |
Gallus gallus | ethyllactate + NADP+ | - |
? | |
isatin + NADPH | s-cis configuration of the neighbouring carbonyls is important for activity | Gallus gallus | ? | - |
? | |
methyl pyruvate + NADPH | - |
Gallus gallus | methyllactate + NADP+ | - |
? | |
Phenanthrenequinone + NADPH | s-cis configuration of the neighbouring carbonyls is important for activity | Gallus gallus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6 | diacetyl + NADPH | Gallus gallus |
6 | 6.8 | diacetyl + NADH | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | oxidation of acetoin to 2,3-butanediol | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Gallus gallus | |
NADP+ | - |
Gallus gallus | |
NADPH | - |
Gallus gallus |