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Literature summary for 1.1.1.162 extracted from
- D-Erythrulose reductase from beef liver (1982), Methods Enzymol., 89, 232-237.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| NADP+ protects against heat inactivation or Rose bengal-sensitized photoinactivation and cold inactivation | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',5'-ADP | - |
Bos taurus |  |
| 2'-AMP | - |
Bos taurus | |
| NADP+ | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0068 | - |
NADPH | - |
Bos taurus | |
| 0.22 | - |
NADH | - |
Bos taurus | |
| 0.36 | - |
D-erythrulose | with NADH | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
4 * 22000, SDS-PAGE | Bos taurus |
| 90000 | - |
sedimentation equilibrium analysis, gel filtration, sucrose density gradient centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| photooxidation, in presence of Rose bengal, protection by NADP+ and to a lesser extent by 2',5'-ADP | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-threitol + NADP+ = D-erythrulose + NADPH + H+ | in an early reference the enzymatic reaction product of D-erythrulose was incorrectly identified as erythritol, gas-liquid and thin-layer chromatographic data have since confirmed the product to be D-threitol, not erythritol, for this reason the systematic name D-threitol:NADP+ oxidoreductase is now used instead of erythritol:NADP+ oxidoreductase | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
| kidney | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
| pancreas | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71.3 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, as crystalline suspension in 1.43 mM ammonium sulfate, pH 8.2, 15 mM dithiothreitol, 0.2 mM NADP+, several months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythrulose + NAD(P)H | highly specific for D-erythrulose | Bos taurus | D-threitol + NAD(P)+ | in reference 2 the enzymatic reaction product of D-erythrulose is incorrectly identified as erythritol, gas-liquid and thin-layer chromatographic data have confirmed the product to be D-threitol, not erythritol, for this reason the systematic name D-threitol:NADP+ oxidoreductase should be used instead of erythritol:NADP+ oxidoreductase which has been designated by the IUB Enzyme commission | r | |
| D-erythrulose + NAD(P)H | the equilibrium of the reaction strongly favors the reduction of D-erythrulose, the reverse reaction is slightly detectable when carried out at pH 7.5-9.0 using NADP+, erythritol does not serve as substrate | Bos taurus | D-threitol + NAD(P)+ | in reference 2 the enzymatic reaction product of D-erythrulose is incorrectly identified as erythritol, gas-liquid and thin-layer chromatographic data have confirmed the product to be D-threitol, not erythritol, for this reason the systematic name D-threitol:NADP+ oxidoreductase should be used instead of erythritol:NADP+ oxidoreductase which has been designated by the IUB Enzyme commission | r | |
| D-erythrulose + NADH + H+ | - |
Bos taurus | D-threitol + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 22000, SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
cold inactivation | Bos taurus |
| additional information | - |
cold inactivation accelerated by increasing salt concentration and decreasing enzyme concentration | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.85 | - |
D-erythrulose + NADH | Bos taurus |
| 6.25 | - |
D-erythrulose + NADH | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | utilized more slowly than NADPH | Bos taurus | |
| NADP+ | enzyme contains 2-3 mol of bound NADP+ | Bos taurus | |
| NADPH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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