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Literature summary for 1.1.1.159 extracted from

  • Pedrini, P.; Andreotti, E.; Guerrini, A.; Dean, M.; Fantin, G.; Giovannini, P.P.
    Xanthomonas maltophilia CBS 897.97 as a source of new 7beta- and 7alpha-hydroxysteroid dehydrogenases and cholylglycine hydrolase: improved biotransformations of bile acids (2006), Steroids, 71, 189-198.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is useful as biocatalyst in the reduction of 7-keto bile acids, co-operation with cholylglycine hydrolase, EC 3.5.1.24, overview Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
NAD+ pH 7.5, 22°C Stenotrophomonas maltophilia
1
-
cholic acid pH 7.5, 22°C Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Stenotrophomonas maltophilia substrate specificity and cycles in bile acid pathways, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia CBS 897.97
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 175fold by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction and affinity chromatography Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Stenotrophomonas maltophilia
70
-
purified native enzyme Stenotrophomonas maltophilia

Storage Stability

Storage Stability Organism
4°C, purified enzyme, 50 mM sodium phosphate, pH 7.5, stable for 2 days Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3alpha,5beta)-3-hydroxy-7,12-dioxocholan-24-oic acid + NADH
-
Stenotrophomonas maltophilia (3alpha,5beta,7alpha)-3,7-dihydroxy-12-oxocholan-24-oic acid + NAD+
-
r
3,7-diketocholic acid + NADH high activity Stenotrophomonas maltophilia 3-ketocholic acid + NAD+
-
r
6-fluoro-7-ketohyocholic acid + NADH
-
Stenotrophomonas maltophilia 6-fluoro-3alpha,6alpha,7alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ + NAD+
-
r
7-ketochenodeoxycholic acid + NADH
-
Stenotrophomonas maltophilia 3alpha,7alpha-dihydroxy-5beta-cholanoic acid + NAD+
-
r
7-ketohyocholic acid + NADH low activity Stenotrophomonas maltophilia 3alpha,6alpha,7alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+
-
r
cholic acid + NADH low activity in the reverse reaction Stenotrophomonas maltophilia 7-ketocholic acid + NAD+
-
r
additional information substrate specificity and cycles in bile acid pathways, overview Stenotrophomonas maltophilia ?
-
?

Synonyms

Synonyms Comment Organism
7alpha-HSDH
-
Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
substrate cholic acid Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Stenotrophomonas maltophilia

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Stenotrophomonas maltophilia
NADH
-
Stenotrophomonas maltophilia