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Literature summary for 1.1.1.156 extracted from

  • Amotz-Ben, A.; Avron, M.
    NADP specific dihydroxyacetone reductase from Dunaliella parva (1973), FEBS Lett., 29, 153-155.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADPH
-
Dunaliella parva
0.015
-
NADP+
-
Dunaliella parva
0.8
-
dihydroxyacetone
-
Dunaliella parva
1400
-
glycerol
-
Dunaliella parva

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NADP+ Dunaliella parva low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell dihydroxyacetone + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Dunaliella parva
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:dihydroxyacetone-grown cell
-
Dunaliella parva
-
culture condition:glycerol-grown cell
-
Dunaliella parva
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-ethanediol + NADP+ 0.3% of the activity compared to glycerol Dunaliella parva hydroxyacetaldehyde + NADPH
-
r
1,2-propanediol + NADP+ 10% of the activity compared to glycerol Dunaliella parva hydroxyacetone + NADPH
-
r
2,3-butanediol + NADP+ 12% of the activity compared to glycerol Dunaliella parva 3-hydroxybutan-2-one + NADPH
-
r
dihydroxyacetonephosphate + NADPH very low activity Dunaliella parva glycerol 3-phosphate + NADP+
-
?
glycerol + NADP+ low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell Dunaliella parva dihydroxyacetone + NADPH + H+
-
r
iso-erythritol + NADP+ 0.9% of the activity compared to glycerol Dunaliella parva ? + NADPH
-
r
L-glyceraldehyde + NADPH very low activity Dunaliella parva L-glycerol + NADP+
-
?
L-ribulose + NADPH very low activity Dunaliella parva ? + NADP+
-
?
meso-erythritol + NADP+ 0.4% of the activity compared to glycerol Dunaliella parva ? + NADPH
-
r
methylglyoxal + NADPH very low activity Dunaliella parva 2-hydroxypropanal + NADP+
-
?
sedoheptulose + NADPH very low activity Dunaliella parva ? + NADP+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
dihydroxyacetone reduction Dunaliella parva
9
-
glycerol oxidation Dunaliella parva

Cofactor

Cofactor Comment Organism Structure
NADP+ can not be replaced by NAD+ Dunaliella parva