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Literature summary for 1.1.1.154 extracted from
- S-ureidoglycolate dehydrogenase: Purification and properties (1971), Arch. Biochem. Biophys., 145, 465-469.
General Stability
| General Stability | Organism |
|---|---|
| incubation with 30% glycerol at 4°C for 3 h stabilizes activity | Arthrobacter allantoicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycolate | 18% inhibition at 17 mM | Arthrobacter allantoicus |  |
| glyoxylate | non-competitive inhibition, Ki: 83 mM | Arthrobacter allantoicus | |
| L-lactate | 10% inhibition at 17 mM | Arthrobacter allantoicus | |
| Zn2+ | 40% inhibition at 0.5 mM | Arthrobacter allantoicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
NADP+ | - |
Arthrobacter allantoicus | |
| 2.16 | - |
NAD+ | - |
Arthrobacter allantoicus | |
| 9.1 | - |
(R,S)-ureidoglycolate | - |
Arthrobacter allantoicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-ureidoglycolate + NAD(P)+ | Arthrobacter allantoicus | strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals | oxalureate + NAD(P)H | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter allantoicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Arthrobacter allantoicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Arthrobacter allantoicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.6 | - |
- |
Arthrobacter allantoicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R,S)-ureidoglycolate + NAD+ | - |
Arthrobacter allantoicus | oxalureate + NADH + H+ | - |
r | |
| (S)-ureidoglycolate + NAD(P)+ | strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals | Arthrobacter allantoicus | oxalureate + NAD(P)H | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
unstable above | Arthrobacter allantoicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.4 | sensitive to ionic strength | Arthrobacter allantoicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
decrease of activity below | Arthrobacter allantoicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Arthrobacter allantoicus | |
| NADP+ | - |
Arthrobacter allantoicus | |
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Release 2023.1 (January 2023)
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