Literature summary for 1.1.1.153 extracted from

Supangat, S.; Choi, Y.K.; Park, Y.S.; Son, D.; Han, C.D.; Lee, K.H.
Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum (2005), Acta Crystallogr. Sect. F, 61, 202-204.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged native and selenomethionine-labeled enzyme in Escherichia coli strain BL21(DE3)	Chlorobaculum tepidum

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified native and selenomethionine-labeled enzyme, hanging drop vapour diffusion microbatch method, 18°C, 0.004 ml protein solution containing 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 0.001 ml of a mixture of 0.004 ml or reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 34% PEG 400, and 0.001 ml additive solution 1 M guanidinium hydrochloride, 2 days, X-ray diffraction structure determination and analysis at 2.1 A resolution	Chlorobaculum tepidum

Crystallization (Comment)

Organism

purified native and selenomethionine-labeled enzyme, hanging drop vapour diffusion microbatch method, 18°C, 0.004 ml protein solution containing 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 0.001 ml of a mixture of 0.004 ml or reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 34% PEG 400, and 0.001 ml additive solution 1 M guanidinium hydrochloride, 2 days, X-ray diffraction structure determination and analysis at 2.1 A resolution

Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+	Chlorobaculum tepidum	last step of the de novo tetrahydrobiopterin biosynthesis initiating from GTP	L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlorobaculum tepidum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged native and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration	Chlorobaculum tepidum

Reaction

Reaction	Comment	Organism	Reaction ID
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+	active site structure	Chlorobaculum tepidum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+	last step of the de novo tetrahydrobiopterin biosynthesis initiating from GTP	Chlorobaculum tepidum	L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+	-	?
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+	two reductive steps oxidizing NADPH	Chlorobaculum tepidum	L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
SR	-	Chlorobaculum tepidum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Chlorobaculum tepidum