Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.153 extracted from

  • Cho, S.H.; Na, J.U.; Youn, H.; Hwang, C.S.; Lee, C.H.; Kang, S.O.
    Sepiapterin reductase producing L-threo-dihydrobiopterin from Chlorobium tepidum (1999), Biochem. J., 340, 497-503.
No PubMed abstract available
Search for more literature for 1.1.1.153

Inhibitors

Inhibitors Comment Organism Structure
Melatonin very slight Chlorobaculum tepidum
N-acetyldopamine very slight Chlorobaculum tepidum
N-acetylserotonin very slight Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 NADPH
-
 Chlorobaculum tepidum
0.021
-
 sepiapterin
-
 Chlorobaculum tepidum
4
-
 diacetyl
-
 Chlorobaculum tepidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, gradient SDS-PAGE Chlorobaculum tepidum
55000
-
 gel filtration Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(S)-lactoyl-7,8-dihydropterin + NADPH Chlorobaculum tepidum
-
 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlorobaculum tepidum

Reaction

Reaction Comment Organism Reaction ID
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ enzyme also reduces carbonyl compounds of non-pteridine derivatives Chlorobaculum tepidum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.5
-
-
 Chlorobaculum tepidum

Storage Stability

Storage Stability Organism
-70°C, in 20 mM Tris-HCl, pH 8.8 Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Chlorobaculum tepidum 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
diacetyl + NADPH
-
 Chlorobaculum tepidum ?
-
 ?
dihydrotepidopterin + NADP+ 15% relative activity Chlorobaculum tepidum ?
-
 ?
L-erythro-dihydrobiopterin + NADP+
-
 Chlorobaculum tepidum ?
-
 ?
L-threo-dihydrobiopterin + NADP+ D-isomer 45% relative activity Chlorobaculum tepidum ?
-
 r
L-threo-dihydroneopterin + NADP+
-
 Chlorobaculum tepidum ?
-
 ?
additional information not: methylglyoxal, menadione Chlorobaculum tepidum ?
-
 ?
phenylglyoxal + NADPH
-
 Chlorobaculum tepidum ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 26000, gradient SDS-PAGE Chlorobaculum tepidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 highest activity, activity decreases slowly above Chlorobaculum tepidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 relativley stable for 2 h in this range, loss of activity in 20 min at 70°C Chlorobaculum tepidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 in Tris-HCl buffer or in glycine-NaOH buffer Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
additional information does not show any activity with NADH Chlorobaculum tepidum