Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition by specific antibody from rabbits | Rattus norvegicus | |
N-acetylserotonin | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.002 | - |
pyruvoyltetrahydropterin | pH 7.4, 37°C | Rattus norvegicus | |
0.007 | - |
1'-hydroxy-2'-oxopropyltetrahydropterin | pH 7.4, 37°C | Rattus norvegicus | |
0.008 | - |
lactoyltetrahydropterin | pH 7.4, 37°C | Rattus norvegicus | |
0.009 | - |
sepiapterin | pH 7.4, 37°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
2 * 27500, SDS-PAGE | Rattus norvegicus |
56000 | - |
second band in SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | terminal step in the biosynthetic pathway for tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
native enzyme from erythrocytes by hydroxylapatite and NADP+-affinity chromatography, to homogeneity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
blood | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
erythrocyte | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1'-hydroxy-2'-oxopropyltetrahydropterin + NADP+ | - |
Rattus norvegicus | L-erythro-tetrahydrobiopterin + NADPH | - |
? | |
1'-hydroxy-2'-oxopropyltetrahydropterin + NADPH | - |
Rattus norvegicus | L-erythrotetrahydrobiopterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | terminal step in the biosynthetic pathway for tetrahydrobiopterin | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-(S)-lactoyl-7,8-dihydropterin + NADPH | catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | - |
Rattus norvegicus | L-erythro-tetrahydrobiopterin + 2 NADP+ | - |
? | |
lactoyltetrahydropterin + NADPH | - |
Rattus norvegicus | L-erythrotetrahydrobiopterin + NADP+ | - |
? | |
lactoyltetrahydropterin + NADPH + H+ | - |
Rattus norvegicus | L-erythro-tetrahydrobiopterin + NADP+ | - |
? | |
additional information | descending substrate preference: sepiapterin, lactoyltetrahydropterin, pyruvoyltetrahydropterin, 1'-hydroxy-2'-oxopropyltetrahydropterin | Rattus norvegicus | ? | - |
? | |
pyruvoyltetrahydropterin + NADPH | - |
Rattus norvegicus | L-erythrotetrahydrobiopterin + NADP+ | - |
? | |
sepiapterin + NADPH + H+ | best substrate | Rattus norvegicus | dihydrobiopterin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Rattus norvegicus |
homodimer | 2 * 27500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |