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Literature summary for 1.1.1.149 extracted from

  • Couture, J.F.; Cantin, L.; Legrand, P.; Luu-The, V.; Labrie, F.; Breton, R.
    Expression, crystallization and preliminary X-ray analysis of human and rabbit 20alpha-hydroxysteroid dehydrogenases in complex with NADP(H) and various steroid substrates (2002), Acta Crystallogr. Sect. D, 58, 135-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli Homo sapiens
expression of soluble, active enzyme as GST-fusion enzyme in Escherichia coli Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NADP+ and progesterone Homo sapiens
in complex with NADP+ and progesterone, with NADP+ and dihydrotestosterone, with NADP+ and 4-androstenedione Oryctolagus cuniculus
purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution Oryctolagus cuniculus
purified recombinant enzyme in complex with cofactor and several substrates, hanging drop vapour diffusion method, 4°C, HEPES or sodium acetate buffer, CaCl2, precipitant is PEG 4000, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17alpha-hydroxyprogesterone + NAD(P)H Homo sapiens
-
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
r
17alpha-hydroxyprogesterone + NAD(P)H Oryctolagus cuniculus
-
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity Homo sapiens
recombinant GST-fusion enzyme by glutahione affinity chromatography and cleavage of the GST-tag by Factor Xa, followed by ion exchange chromatography, and gel filtration, to homogeneity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + NAD(P)H
-
Homo sapiens 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
r
17alpha-hydroxyprogesterone + NAD(P)H
-
Oryctolagus cuniculus 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+
-
r

Synonyms

Synonyms Comment Organism
20alpha-HSD
-
Homo sapiens
20alpha-HSD
-
Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens
7.5
-
assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Homo sapiens
NAD(P)+
-
Oryctolagus cuniculus
NAD(P)H
-
Homo sapiens
NAD(P)H
-
Oryctolagus cuniculus