Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition but increase at preincubation with antiserum | Rattus norvegicus | |
prolactin | down-regulating of gene-expression | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female, pregnant, Sprague-Dawley | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
solubilized, separated by SDS-PAGE, protein extraction from gel, renatured | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
6 M guanidine hydrochloride, 20 mM Tris-HCl buffer, pH 7.4, 100 mM KCl, 10% glycerol, 1 mM DTT, 1 h, 25°C | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | corpus luteum | Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-80°C, particulate and cytosolic fraction after homogenization | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20alpha-dihydroprogesterone + NADP+ | - |
Rattus norvegicus | progesterone + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
15 min, almost total loss of activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |