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Literature summary for 1.1.1.149 extracted from
- Expression, purification and characterization of the rat luteal 20alpha-hydroxysteroid dehydrogenase (1997), Endocrinology, 138, 182-190.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha and baculovirus-insect cell systems Sf9 | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0058 | 0.0059 | 20alpha-hydroxyprogesterone | 37°C, 10 min | Rattus norvegicus |  |
| 0.0095 | 0.0096 | NADP+ | 37°C, 7.5 min | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| progesterone + NADPH | Rattus norvegicus | central role in luteolysis and parturition | 20alpha-hydroxyprogesterone + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme expressed by insect cells, not essential for catalytic activity | Rattus norvegicus |
| phosphoprotein | enzyme expressed by insect cells, enzyme expressed by E. coli phosphorylated by various kinases, not essential for catalytic activity | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity binding to glutathione-Sepharose and thrombin digestion of bacterial lysate, ion exchange and affinity chromatography of enzyme produced by insect cells | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | corpus luteum | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2596 | - |
insect cells | Rattus norvegicus |
| 0.332 | - |
+/-0.047, Escherichia coli | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxyprogesterone + NADPH + H+ | - |
Rattus norvegicus | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+ | - |
? | |
| 4-nitrobenzaldehyde + NADPH | - |
Rattus norvegicus | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
| 9,10-phenanthrenequinone + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
| benzaldehyde + NADPH | - |
Rattus norvegicus | benzyl alcohol + NADP+ | - |
? | |
| D-galactose + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
| D-glucose + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
| DL-glyceraldehyde + NADPH | - |
Rattus norvegicus | glycerol + NADP+ | - |
? | |
| methylglyoxal + NADPH | - |
Rattus norvegicus | NADP+ + ? | - |
? | |
| additional information | benzaldehyde, DL-glyceraldehyde, 4-nitrobenzaldehyde and glucose very efficient substrates, methylglyoxal, 9,10-phenanthrenquinone and D-galactose less active, no activity with corticosterone and 5alpha-dihydrotestosterone | Rattus norvegicus | ? | - |
? | |
| progesterone + NADPH | - |
Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
r | |
| progesterone + NADPH | central role in luteolysis and parturition | Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, SDS-PAGE | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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