General Stability | Organism |
---|---|
NADP+ protects against thermal denaturation, steroid substrate does not | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',5'-adenosine bisphosphate | competitive inhibition versus NADP+ | Rattus norvegicus | |
2',5'-ADP | coenzyme-competitive inhibitor | Rattus norvegicus | |
2'-adenylic acid | coenzyme-competitive inhibitor | Rattus norvegicus | |
2'-phosphoadenosine diphosphoribose | coenzyme-competitive inhibitor | Rattus norvegicus | |
3',5'-cyclic AMP | coenzyme-competitive inhibitor | Rattus norvegicus | |
3-Aminopyridine 1,N6-ethenoadenine dinucleotide phosphate | NADP analog, competitive inhibitor | Rattus norvegicus | |
3-Aminopyridine adenine dinucleotide phosphate | NADP analog, competitive inhibitor | Rattus norvegicus | |
5'-adenosine diphosphate | - |
Rattus norvegicus | |
5'-Adenosine triphosphate | - |
Rattus norvegicus | |
5'-adenylic acid | coenzyme-competitive inhibitor | Rattus norvegicus | |
5'-ADP | coenzyme-competitive inhibitor | Rattus norvegicus | |
5'-ATP | coenzyme-competitive inhibitor | Rattus norvegicus | |
adenosine 2'-monophospho- 5'-diphosphoribose | strong inhibition | Rattus norvegicus | |
adenosine diphosphoribose | coenzyme-competitive inhibitor | Rattus norvegicus | |
aminopyridine 1,N6-ethenoadenine dinucleotide phosphate | - |
Rattus norvegicus | |
aminopyridine adenine dinucleotide phosphate | - |
Rattus norvegicus | |
carboxylic acid | competitive for acetic and propionic acid, mixed-type from pentanoic to undecanoic acid with exception of heptanoic acid | Rattus norvegicus | |
Diazotized 3-aminopyridine 1,N6-ethenoadenine dinucleotide phosphate | NADP+ analog, competitive inhibitor | Rattus norvegicus | |
Diazotized 3-aminopyridine adenine dinucleotide phosphate | NADP analog, competitive inhibitor | Rattus norvegicus | |
linear monocarboxylic acids | slight inhibition, overview | Rattus norvegicus | |
linear N-alkylammonium chloride derivatives | slight inhibition, overview | Rattus norvegicus | |
additional information | adenosine: no inhibition, important features of coenzyme structure binding to coenzyme-binding site are 2'-phosphate, diphosphate and the positively charged ring nitrogen, hydrophobic region nearby the NADP-binding site might be responsible for nonpolar faciliation of the rates of maleimide inactivation; no inhibition by ATP, 3,5-cyclic AMP, and by adenosine up to 7.5 mM | Rattus norvegicus | |
N-1-butylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-decylnicotinamide chloride | inhibition of the ovarian enzyme, mixed type inhibition versus NADP+ | Rattus norvegicus | |
N-1-dodecylnicotinamide chloride | inhibition of the ovarian enzyme, mixed type inhibition versus NADP+ | Rattus norvegicus | |
N-1-ethylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-heptylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-hexylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-methylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-nonylnicotinamide chloride | inhibition of the ovarian enzyme, mixed type inhibition versus NADP+ | Rattus norvegicus | |
N-1-octylnicotinamide chloride | inhibition of the ovarian enzyme, mixed type inhibition versus NADP+ | Rattus norvegicus | |
N-1-pentylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-propylnicotinamide chloride | inhibition of the ovarian enzyme, competitive inhibition versus NADP+ | Rattus norvegicus | |
N-1-undecylnicotinamide chloride | inhibition of the ovarian enzyme, mixed type inhibition versus NADP+ | Rattus norvegicus | |
N-alkylammonium chloride | competitive from pentyl to heptyl, mixed type from nonyl to dodecyl | Rattus norvegicus | |
N-alkylmaleimides | coenzyme-competitive inhibition, varying in chainlength from N-ethyl- to N-octylmaleimide, effectively inactivated the enzyme through pseudo-first-order rate processes, overview | Rattus norvegicus | |
N1-alkylnicotinamide chloride | competitive from methyl to heptyl, mixed-type from octyl to dodecyl with respect to NADP | Rattus norvegicus | |
NAD+ | NADP analog, competitive inhibitor | Rattus norvegicus | |
NADP+ | coenzyme-competitive inhibitor | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NAD(P)H | Rattus norvegicus | - |
20alpha-hydroxypregn-4-en-3-one + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, affinity chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ | bibi mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NAD(P)H | - |
Rattus norvegicus | 20alpha-hydroxypregn-4-en-3-one + NAD(P)+ | - |
r | |
progesterone + NAD(P)H | i.e. pregn-4-en-3-one, importance of sulfhydryl groups in the functioning of the enzyme | Rattus norvegicus | 20alpha-hydroxypregn-4-en-3-one + NAD(P)+ | - |
r | |
progesterone + NADPH | - |
Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
20alpha-HSD | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
NADP+ protects against thermal inactivation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Rattus norvegicus | |
NAD(P)H | the 2-phosphate, the diphosphate, and the positively charged ring nitrogen are important features of the coenzyme structure in binding to the coenzyme-binding site of the enzyme | Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
0.0005 | - |
Diazotized 3-aminopyridine adenine dinucleotide phosphate | pH 7.8, 23°C | Rattus norvegicus | |
0.00074 | - |
NADP+ | pH 7.8, 23°C | Rattus norvegicus | |
0.00087 | - |
adenosine 2'-monophospho- 5'-diphosphoribose | pH 7.8, 23°C | Rattus norvegicus | |
0.00134 | - |
2',5'-adenosine bisphosphate | pH 7.8, 23°C | Rattus norvegicus | |
0.0029 | - |
aminopyridine adenine dinucleotide phosphate | pH 7.8, 23°C | Rattus norvegicus | |
0.01 | - |
aminopyridine 1,N6-ethenoadenine dinucleotide phosphate | pH 7.8, 23°C | Rattus norvegicus | |
0.022 | - |
Diazotized 3-aminopyridine 1,N6-ethenoadenine dinucleotide phosphate | pH 7.8, 23°C | Rattus norvegicus | |
0.148 | - |
2'-adenylic acid | pH 7.8, 23°C | Rattus norvegicus | |
0.205 | - |
N-1-methylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
0.34 | - |
ADP | pH 7.8, 23°C | Rattus norvegicus | |
0.487 | - |
N-1-undecylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
0.95 | - |
adenosine diphosphoribose | pH 7.8, 23°C | Rattus norvegicus | |
1.24 | - |
N-1-decylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
2.84 | - |
5'-adenylic acid | pH 7.8, 23°C | Rattus norvegicus | |
3.29 | - |
N-1-nonylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
3.39 | - |
N-1-heptylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
4.01 | - |
N-1-octylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
6.59 | - |
N-1-hexylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
8.24 | - |
N-1-propylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
8.95 | - |
N-1-ethylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
9.29 | - |
N-1-butylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
9.35 | - |
N-1-pentylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus | |
14.8 | - |
N-1-methylnicotinamide chloride | pH 7.8, 23°C | Rattus norvegicus |