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Literature summary for 1.1.1.146 extracted from

  • Skarydova, L.; Skarka, A.; Novotna, R.; Zivna, L.; Martin, H.J.; Wsol, V.; Maser, E.
    Partial purification and characterization of a new human membrane-bound carbonyl reductase playing a role in the deactivation of the anticancer drug oracin (2009), Toxicology, 264, 52-60.
    View publication on PubMed

Application

Application Comment Organism
drug development 11beta-HSD1 will significantly contribute to the biotransformation of oracin in humans. The microsomal carbonyl reductase has a great potential to significantly impair the chemotherapy with the anticancer drug oracin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
oracin
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-
microsome
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilization, partially purifed by gel filtration, 73fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oracin + NADPH + H+ the partially purified enzyme catalyzes the reduction of oracin in a stereospecific manner and with higher efficiency and affinity compared to microsomal 11beta-HSD1. The enzyme purified from human liver reduces oracin to (-)-dihydrooracin and (+)-dihydrooracin in a ratio of 76 to 24. Whole human liver microsomes metabolise oracin with different stereospecificity, where the formation of (-)-dihydrooracin (60%) predominates over (+)-dihydrooracin (40%) Homo sapiens 11-dihydrooracin + NADP+
-
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Synonyms

Synonyms Comment Organism
11beta-HSD1
-
Homo sapiens
11beta-hydroxysteroid dehydrogenase type 1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Homo sapiens