Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.146 extracted from

  • Kim, K.W.; Wang, Z.; Busby, J.; Tsuruda, T.; Chen, M.; Hale, C.; Castro, V.M.; Svensson, S.; Nybo, R.; Xiong, F.; Wang, M.
    The selectivity of tyrosine 280 of human 11beta-hydroxysteroid dehydrogenase type 1 in inhibitor binding (2007), FEBS Lett., 581, 995-999.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression inEscherichia coli of N-terminally deleted gene, amino acids 24-292, with an N-terminal His-tag Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y280A wild-type and mutant enzyme exhibit similar Km values for either cortisone or 11-dehydrocorticosterone. About 10fold increase in IC50-value for glycyrrhetinic acid Homo sapiens
Y280F wild-type and mutant enzyme exhibit similar Km values for either cortisone or 11-dehydrocorticosterone. About 6fold increase in IC50-value for glycyrrhetinic acid Homo sapiens
Y280S wild-type and mutant enzyme exhibit similar Km values for either cortisone or 11-dehydrocorticosterone. 100% increase in IC50-value for glycyrrhetinic acid Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P28845
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-dehydrocorticosterone + NADPH
-
Homo sapiens corticosterone + NADP+
-
r
cortisol + NADP+
-
Homo sapiens cortisone + NADPH
-
r
cortisone + NADPH + H+
-
Homo sapiens cortisol + NADP+
-
r

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens