Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.146 extracted from

  • Zhang, J.; Osslund, T.D.; Plant, M.H.; Clogston, C.L.; Nybo, R.E.; Xiong, F.; Delaney, J.M.; Jordan, S.R.
    Crystal structure of murine 11 beta-hydroxysteroid dehydrogenase 1: an important therapeutic target for diabetes (2005), Biochemistry, 44, 6948-6957.
    View publication on PubMed

Application

Application Comment Organism
pharmacology the enzyme is an important therapeutic target for diabetes in humans Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, 25 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 0.1% Triton, 5% glycerol, and 2 mM EDTA, mixed with equal volume of well solution containing 1.8 M Li2O4, and 0.1 M HEOES, pH 7.5, at room temperature, derivatization with 1 mM ethylmercuric chloride, soaking of crystals with NADH and corticoserone containing solution, X-ray diffraction structure determination and analysis at 3.0 A resolution Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P50172 isozymes 11beta-HSD1 and 11beta-HSD2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration Mus musculus

Reaction

Reaction Comment Organism Reaction ID
an 11beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH + H+ substrate binding site structure, reaction mechanism involving Lys187 and Tyr183, active site structure involving a S-Y-K catalytic triad Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-dehydrocorticosterone + NADPH isozyme 11beta-HSD1 catalyzes the forward reaction with cofactor NADPH Mus musculus corticosterone + NADP+
-
ir
cortisone + NADPH + H+ isozyme 11beta-HSD1 catalyzes the forward reaction with cofactor NADPH Mus musculus cortisol + NADP+
-
ir

Synonyms

Synonyms Comment Organism
11beta-HSD1
-
Mus musculus
11beta-hydroxysteroid dehydrogenase 1
-
Mus musculus