Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L150R | also lacking the first 30 n-terminal amino acids, gains activity in microsomal fraction after solubilization with Emulgen | Homo sapiens |
V148R | also lacking the first 30 N-terminal amino acids, no activity | Homo sapiens |
V149E | also lacking the first 30 N-terminal amino acids, no activity | Homo sapiens |
V149R | also lacking the first 30 N-terminal amino acids, active and soluble in cytosol, gains activity in microsomal fraction after solubilization with Emulgen | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
cortisol | 37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1 | Homo sapiens | |
0.1 | - |
cortisone | 37°C, pH 7.4, V149R mutant 11beta-HSD1, V149E mutant 11beta-HSD1, and N-terminal 15 amino acid truncated V149R and V149E mutant 11beta-HSD1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
membrane | anchored within the endoplasmic reticulum membrane with one single N-terminal hydrophobic domain | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | Homo sapiens | - |
cortisol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3 different glycosylation sites | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | - |
Homo sapiens | cortisol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Homo sapiens |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Homo sapiens |