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Literature summary for 1.1.1.146 extracted from

  • Agarwal, A.K.; Tusie-Luna, M.T.; Monder, C.; White, P.C.
    Expression of 11beta-hydroxysteroid dehydrogenase using recombinant vaccinia virus (1990), Mol. Endocrinol., 4, 1827-1832.
    View publication on PubMed
Search for more literature for 1.1.1.146

Activating Compound

Activating Compound Comment Organism Structure
Triton DF-18 slight activation Rattus sp.
Triton X-100 slight activation Rattus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in TK-143 B cells by vaccinia virus infection Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate decreased dehydrogenase activity, no effect on reductase activity; i.e. CHAPS Rattus sp.
3beta-hydroxy-11-oxo-18beta-olean-12-en-30-oic-acid 50% inhibition of dehydrogenase activity at 5 nM, 50% inhibition of oxoreductase activity at 20 nM; i.e. glycyrrhetinic acid Rattus sp.
A1-tunicamycin 50% inhibition at 50 ng/ml, reductase activity not affected Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 7.0 Rattus sp.
0.00144
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 7.0 Rattus sp.
0.00214
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 8.5 Rattus sp.
0.00283
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 8.5 Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ Rattus sp.
-
 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+
-
 Rattus sp. 21-hydroxy-pregn-4-en-3,11,20-trione + NADPH + H+
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 pH 7.0 or 8.5, stable for 1 h Rattus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000883
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 7.0 Rattus sp.
0.000113
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 7.0 Rattus sp.
0.000183
-
 21-hydroxy-pregn-4-en-3,11,20-trione at pH 8.5 Rattus sp.
0.000228
-
 11beta,21-dihydroxypregn-4-en-3,20-dione at pH 8.5 Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 reductase activity Rattus sp.
8.5 9.5 oxidase activity Rattus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 11-dehydrogenase activity stable for 2 h, reductase activity rapidly decreases Rattus sp.
8.5
-
 11-dehydrogenase activity stable for 2 h, reductase activity rapidly decreases Rattus sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rattus sp.
NADPH
-
 Rattus sp.