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Literature summary for 1.1.1.145 extracted from

  • Zhao, Y.; Xiao, J.
    Homology modeling and molecular dynamics simulation studies of human type 1 3beta-hydroxysteroid dehydrogenase: Toward the understanding of cofactor specificity (2011), J. Comput. Chem., 32, 33-42.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D35A/K36R the D35A/K36R mutant enzyme of 3beta-HSD1 has no measurable 3beta-HSD activity with NADH as the cofactor and now uses NADPH as the cofactor and NADH specificity of the wild-type 1 isomerase is completely lost Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14060
-
-

Synonyms

Synonyms Comment Organism
3beta-HSD1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ NADH forms hydrogen bond with Asp35 in the NADH-wild-type 3beta-HSD1 complex Homo sapiens
NADP+ NADPH forms hydrogen bond with Lys36 in the NADPH-wild-type 3beta-HSD1 complex. The total contribution of Asp35 and Lys36 is most favorable in the case of NADPH binding to the wild-type enzyme when compared with NADH, and Lys36 have strong electronic interaction with NADPH Homo sapiens