Cloned (Comment) | Organism |
---|---|
expressed in Sf9 cells using the baculovirus system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trilostane | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Homo sapiens | 5743 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | using fluorescence resonance energy transfer, it is shown that 3betaHSD2 interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P26439 | - |
- |
Renatured (Comment) | Organism |
---|---|
enzyme interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine. It becomes less stable when incubated with the individual lipids. Dipalmitoylphosphatidylglycerol, alone or in combination with dipalmitoylphosphatidylcholine, leads to a decrease in alpha-helical content without an effect on the beta-sheet conformation. With the exception of the 20 N-terminal amino acids, mixed vesicles protect the enzyme from trypsin digestion. Protein incubated with dipalmitoylphosphatidylcholine is only partially protected | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | using fluorescence resonance energy transfer, it is shown that 3betaHSD2 interacts strongly with a mixture of dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine | Homo sapiens | ? | - |
? | |
additional information | enzyme diplays lipid-mediated unfolding. As isoform 3betaHSD2 lacks a receptor, opening the conformation may activate the protein by forming a cavity forms between the alpha-helix and beta-sheet | Homo sapiens | ? | - |
? | |
pregnenolone + NAD+ | - |
Homo sapiens | progesterone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3betaHSD2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
3betaHSD2 becomes less stable when incubated with the individual lipids dipalmitoylphosphatidylglycerol or dipalmitoylphosphatidylcholine, as indicated by the decrease in thermal denaturation (Tm) from 42 to 37°C. Dipalmitoylphosphatidylglycerol alone or in combination with dipalmitoylphosphatidylcholine, leads to a decrease in alpha-helical content without an effect on the beta-sheet conformation | Homo sapiens |
37 | - |
approximately, melting temperature in the presence of zwitterionically charged dipalmitoylphosphatidylcholine vesicles or an equal mixture of zwitterionic vesicles and anionic vesicles | Homo sapiens |
42 | - |
melting temperature in the absence of lipid | Homo sapiens |
42 | - |
Tm in the absence of any lipid | Homo sapiens |
65 | - |
complete denaturation | Homo sapiens |