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Literature summary for 1.1.1.145 extracted from

  • Yang, X.; Dubnau, E.; Smith, I.; Sampson, N.S.
    Rv1106c from Mycobacterium tuberculosis is a 3beta-hydroxysteroid dehydrogenase (2007), Biochemistry, 46, 9058-9067.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.145

Cloned(Commentary)

Cloned (Comment) Organism
expression in Mycobacterium smegmatis Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information disruption of gene Rv1106c abrogates bacterial cholesterol oxidation activity Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
NADH mixed type inhibition Mycobacterium tuberculosis
trilostane competitive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 pregnenolone pH 8.5, 37°C Mycobacterium tuberculosis
0.172
-
 NAD+ cosubstrate pregnenolone, pH 8.5, 37°C Mycobacterium tuberculosis
0.221
-
 dehydroepiandrosterone pH 8.5, 37°C Mycobacterium tuberculosis
0.27
-
 NAD+ cosubstrate dehydroepiandrosterone, pH 8.5, 37°C Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Mycobacterium tuberculosis 5622
-

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 product of Rv1106c
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ enzyme forms a ternary complex with NAD+ binding before sterol binding Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + NAD+
-
 Mycobacterium tuberculosis cholest-5-en-3-one + NADH
-
 ?
dehydroepiandrosterone + NAD+
-
 Mycobacterium tuberculosis androst-5-en-3,17-dione + NADH + H+
-
 ?
pregnenolone + NAD+
-
 Mycobacterium tuberculosis progesterone + NADH + H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.48
-
 pregnenolone pH 8.5, 37°C Mycobacterium tuberculosis
11.6
-
 dehydroepiandrosterone pH 8.5, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.197
-
 trilostane pH 8.5, 37°C Mycobacterium tuberculosis
0.245
-
 NADH pH 8.5, 37°C Mycobacterium tuberculosis