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Literature summary for 1.1.1.145 extracted from
- Purification and kinetic properties of 3beta-hydroxysteroid dehydrogenase from bovine adrenocortical microsomes (1985), J. Biochem., 98, 1519-1526.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 17alpha-hydroxypregnenolone | competitive inhibition | Bos taurus |  |
| diethyldicarbonate | - |
Bos taurus | |
| p-chloromercuribenzoate | - |
Bos taurus | |
| pregnenolone | competitive inhibition | Bos taurus | |
| trilostane | i.e. 4alpha,5-epoxy-3,17beta-dihydroxy-5alpha-androst-2-en-2-carbonitrile | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
pregnenolone | - |
Bos taurus | |
| 0.0053 | - |
3beta,17alpha-dihydroxy-5-pregen-20-one | - |
Bos taurus | |
| 0.0062 | 0.0066 | NAD+ | - |
Bos taurus | |
| 4.9 | - |
NADP+ | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pregnenolone + NAD(P)+ | Bos taurus | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.677 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 0.1 M potassium phosphate buffer, pH 7.4, 10 mg protein/ml, at least 3 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Bos taurus | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
| pregnenolone + NAD(P)+ | - |
Bos taurus | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.5 | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | cysteinyl and histidyl at NAD+-binding site | Bos taurus | |
| NADP+ | low activity | Bos taurus | |
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