Literature summary for 1.1.1.141 extracted from

Cho, H.; Oliveira, M.A.; Tai, H.H.
Critical residues for the coenzyme specificity of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (2003), Arch. Biochem. Biophys., 419, 139-146.

Search for more literature for 1.1.1.141

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A13G/A14S/Q15K/D36S/W37R	no activity	Homo sapiens
A14S/Q15K/D36S/W37R	no activity	Homo sapiens
Q15K	approx. 200% of wild-type activity	Homo sapiens
Q15K/D36A/W37R	no activity with NAD+ as cofactor, low activity with NADP+ as cofactor	Homo sapiens
Q15K/D36S/W37R	no activity with NAD+ as cofactor, low activity with NADP+ as cofactor	Homo sapiens
Q15K/W37K	approx. 350% of wild-type activity	Homo sapiens
Q15K/W37R	approx. 350% of wild-type activity, strong activity with NADP+ as cofactor	Homo sapiens
Q15R	approx. 100% of wild-type activity	Homo sapiens
Q15R/W37K	approx. 200% of wild-type activity	Homo sapiens
Q15R/W37R	approx. 200% of wild-type activity, strong activity with NADP+ as cofactor	Homo sapiens
W37K	approx. 100% of wild-type activity	Homo sapiens
W37R	approx. 100% of wild-type activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0063	-	(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate	37°C, pH 7.5	Homo sapiens
0.013	-	(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate	37°C, pH 7.5, Q15K/W37K double mutant	Homo sapiens
0.026	-	(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate	37°C, pH 7.5, Q15K mutant	Homo sapiens
0.032	-	NAD+	37°C, pH 7.5	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+	Homo sapiens	-	(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant 15-PGDH	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
placenta	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+	-	Homo sapiens	(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
15-PGDH	-	Homo sapiens

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Release 2023.1 (January 2023)