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Literature summary for 1.1.1.141 extracted from

  • Bergholte, J.M.; Okita, R.T.
    Isolation and properties of lung 15-hydroxyprostaglandin dehydrogenase from pregnant rabbits (1986), Arch. Biochem. Biophys., 245, 308-315.
    View publication on PubMed
Search for more literature for 1.1.1.141

Activating Compound

Activating Compound Comment Organism Structure
additional information a 42- to 55-fold induction of the PGDH activity is observed after 20 days of gestation in pregnant rabbits Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuriphenylsulfonic acid
-
 Oryctolagus cuniculus
N-ethylmaleimide
-
 Oryctolagus cuniculus
p-chloromercuriphenylsulfonic acid
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 prostaglandin E1
-
 Oryctolagus cuniculus
0.033
-
 prostaglandin E1 pH 10.2 Oryctolagus cuniculus
0.038
-
 prostaglandin A1
-
 Oryctolagus cuniculus
0.059
-
 prostaglandin E2
-
 Oryctolagus cuniculus
0.059
-
 prostaglandin E2 pH 10.2 Oryctolagus cuniculus
0.066
-
 6-ketoprostaglandin E1
-
 Oryctolagus cuniculus
0.111
-
 6-ketoprostaglandin F1alpha
-
 Oryctolagus cuniculus
0.133
-
 prostaglandin F2alpha
-
 Oryctolagus cuniculus
0.741
-
 Prostaglandin D2
-
 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Oryctolagus cuniculus first step in prostaglandin catabolism (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Oryctolagus cuniculus
-
 pregnant rabbits
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus
native enzyme 2600fold from lung cytosol of pregnant rabbits, by cation and anion exchange chromatography, gel filtration, hydrophobic interaction and hydroxylapatite chromatography to homogeneity Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Oryctolagus cuniculus
-
lung of 28-days-old pregnant rabbits Oryctolagus cuniculus
-
additional information a 42- to 55-fold induction of the PGDH activity is observed after 20 days of gestation in pregnant rabbits Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity during pregnancy, substrate specificity Oryctolagus cuniculus
25
-
-
 Oryctolagus cuniculus
25
-
 purified enzyme Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-80°C, 3-4 weeks Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+
-
 Oryctolagus cuniculus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ first step in prostaglandin catabolism Oryctolagus cuniculus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ i.e. 15-hydroxyprostaglandin or (15S)-PGE2, best substrate Oryctolagus cuniculus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
11alpha,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid + NAD(P)+ specific for NAD+ Oryctolagus cuniculus 11alpha-hydroxy-9,15-diketoprost-5,13-dienoic acid + NADH
-
 ?
6-keto-prostaglandin E1 + NAD+
-
 Oryctolagus cuniculus ? + NADH
-
 ?
6-keto-prostaglandin F1alpha + NAD+
-
 Oryctolagus cuniculus ? + NADH
-
 ?
6-ketoprostaglandin E1 + NAD+
-
 Oryctolagus cuniculus 6,15-diketoprostaglandin E1
-
 ?
6-ketoprostaglandin F1alpha + NAD+
-
 Oryctolagus cuniculus 6,15-diketoprostaglandin F1alpha + NADH + H+
-
 ?
additional information substrate specificity, overview, no activity with prostaglandin B1 Oryctolagus cuniculus ?
-
 ?
prostaglandin A1 + NAD+
-
 Oryctolagus cuniculus 15-ketoprostaglandin A1 + NADH + H+
-
 ?
prostaglandin A1 + NAD+
-
 Oryctolagus cuniculus ? + NADH
-
 ?
prostaglandin D2 + NAD+
-
 Oryctolagus cuniculus 15-ketoprostaglandin D2 + NADH + H+
-
 ?
prostaglandin D2 + NAD+
-
 Oryctolagus cuniculus ? + NADH
-
 ?
prostaglandin E1 + NAD+
-
 Oryctolagus cuniculus ? + NADH
-
 ?
prostaglandin F2alpha + NAD+ specific for NAD+ Oryctolagus cuniculus 15-ketoprostaglandin F2alpha + NADH + H+
-
 ?
prostaglandin F2alpha + NAD+
-
 Oryctolagus cuniculus ? + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
15-hydroxyprostaglandin dehydrogenase
-
 Oryctolagus cuniculus
NAD-dependent 15-hydroxyprostaglandin dehydrogenase
-
 Oryctolagus cuniculus
PGDH
-
 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 10.4
-
 Oryctolagus cuniculus
10 10.4 prostaglandin E1, 3-(cyclohexylamine)propanesulfonic acid buffer Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
9.4 11
-
 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for NAD+ Oryctolagus cuniculus
NAD+ specific for Oryctolagus cuniculus