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Literature summary for 1.1.1.140 extracted from

  • Du Toit, P.J.; Kotze, J.P.
    The isolation and characterization of sorbitol-6-phosphate dehydrogenase from Clostridium pasteurianum (1970), Biochim. Biophys. Acta, 206, 333-342.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NADH high concentrations of Clostridium pasteurianum
sorbitol 6-phosphate
-
Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information overview: various buffers, various substrate concentrations Clostridium pasteurianum
0.02 0.03 NADH phosphate buffer Clostridium pasteurianum
0.04 0.05 sorbitol 6-phosphate Tris-HCl buffer Clostridium pasteurianum
0.07 0.08 NAD+ 0.11-0.45 mM NAD+, Tris-HCl buffer Clostridium pasteurianum
0.19 0.21 NAD+ 0.11-0.45 mM NAD+, glycine-NaOH buffer Clostridium pasteurianum
0.2 0.25 NAD+ 0.45-4.5 mM NAD+, Tris-HCl buffer Clostridium pasteurianum
0.4 0.44 sorbitol 6-phosphate glycine-NaOH buffer Clostridium pasteurianum
0.74 0.84 NAD+ 0.45-4.5 mM NAD+, glycine-NaOH buffer Clostridium pasteurianum
2.8 3 fructose 6-phosphate + 0.43 mM NADH, phosphate buffer Clostridium pasteurianum
7.5 7.8 fructose 6-phosphate + 1.08 mM NADH, phosphate buffer Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000 94000 gel filtration Clostridium pasteurianum

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
176.5 fold purification Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.2
-
-
Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol 6-phosphate + NAD+ high specificity for NAD+ Clostridium pasteurianum D-fructose 6-phosphate + NADH + H+ high specificity for D-fructose 6-phosphate r
D-sorbitol 6-phosphate + NAD+ equilibrium far on the side of sorbitol 6-phosphate formation Clostridium pasteurianum D-fructose 6-phosphate + NADH + H+ high specificity for D-fructose 6-phosphate r
D-sorbitol 6-phosphate + NAD+ high specificity for D-sorbitol 6-phosphate Clostridium pasteurianum D-fructose 6-phosphate + NADH + H+ high specificity for D-fructose 6-phosphate r
mannitol 1-phosphate + NAD+ at 1-5% of reaction with sorbitol 6-phosphate Clostridium pasteurianum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 6.7 reduction of fructose 6-phosphate, phosphate buffer Clostridium pasteurianum
8.5
-
oxidation of sorbitol 6-phosphate, Tris-HCl buffer Clostridium pasteurianum
10
-
oxidation of sorbitol 6-phosphate, glycine-NaOH buffer Clostridium pasteurianum

pH Range

pH Minimum pH Maximum Comment Organism
5.7 7.5 pH 5.7: about 50% of activity maximum, pH 7.5: about 35% of activity maximum Clostridium pasteurianum

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Clostridium pasteurianum
NAD+ specific for Clostridium pasteurianum
NADH specific for Clostridium pasteurianum