Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | high concentrations of | Clostridium pasteurianum | |
sorbitol 6-phosphate | - |
Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview: various buffers, various substrate concentrations | Clostridium pasteurianum | |
0.02 | 0.03 | NADH | phosphate buffer | Clostridium pasteurianum | |
0.04 | 0.05 | sorbitol 6-phosphate | Tris-HCl buffer | Clostridium pasteurianum | |
0.07 | 0.08 | NAD+ | 0.11-0.45 mM NAD+, Tris-HCl buffer | Clostridium pasteurianum | |
0.19 | 0.21 | NAD+ | 0.11-0.45 mM NAD+, glycine-NaOH buffer | Clostridium pasteurianum | |
0.2 | 0.25 | NAD+ | 0.45-4.5 mM NAD+, Tris-HCl buffer | Clostridium pasteurianum | |
0.4 | 0.44 | sorbitol 6-phosphate | glycine-NaOH buffer | Clostridium pasteurianum | |
0.74 | 0.84 | NAD+ | 0.45-4.5 mM NAD+, glycine-NaOH buffer | Clostridium pasteurianum | |
2.8 | 3 | fructose 6-phosphate | + 0.43 mM NADH, phosphate buffer | Clostridium pasteurianum | |
7.5 | 7.8 | fructose 6-phosphate | + 1.08 mM NADH, phosphate buffer | Clostridium pasteurianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | 94000 | gel filtration | Clostridium pasteurianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
176.5 fold purification | Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43.2 | - |
- |
Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol 6-phosphate + NAD+ | high specificity for NAD+ | Clostridium pasteurianum | D-fructose 6-phosphate + NADH + H+ | high specificity for D-fructose 6-phosphate | r | |
D-sorbitol 6-phosphate + NAD+ | equilibrium far on the side of sorbitol 6-phosphate formation | Clostridium pasteurianum | D-fructose 6-phosphate + NADH + H+ | high specificity for D-fructose 6-phosphate | r | |
D-sorbitol 6-phosphate + NAD+ | high specificity for D-sorbitol 6-phosphate | Clostridium pasteurianum | D-fructose 6-phosphate + NADH + H+ | high specificity for D-fructose 6-phosphate | r | |
mannitol 1-phosphate + NAD+ | at 1-5% of reaction with sorbitol 6-phosphate | Clostridium pasteurianum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Clostridium pasteurianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.7 | reduction of fructose 6-phosphate, phosphate buffer | Clostridium pasteurianum |
8.5 | - |
oxidation of sorbitol 6-phosphate, Tris-HCl buffer | Clostridium pasteurianum |
10 | - |
oxidation of sorbitol 6-phosphate, glycine-NaOH buffer | Clostridium pasteurianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 7.5 | pH 5.7: about 50% of activity maximum, pH 7.5: about 35% of activity maximum | Clostridium pasteurianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Clostridium pasteurianum | |
NAD+ | specific for | Clostridium pasteurianum | |
NADH | specific for | Clostridium pasteurianum |