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Literature summary for 1.1.1.14 extracted from

  • Hellgren, M.; Kaiser, C.; de Haij, S.; Norberg, A.; Hoeoeg, J.O.
    A hydrogen-bonding network in mammalian sorbitol dehydrogenase stabilizes the tetrameric state and is essential for the catalytic power (2007), Cell. Mol. Life Sci., 64, 3129-3138.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y110F mutation in hydrogen onding network, complete loss of activity and destabilization of protein into tetramers, dimers and monomers compared to only tetramers for wild-type Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
D-xylitol wild-type, pH 10.0 Rattus norvegicus
0.33
-
D-sorbitol wild-type, pH 10.0 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
plus 80000, 160000 and 300000, mutant Y110F Rattus norvegicus
80000
-
plus 160000, 40000 and 300000, mutant Y110F Rattus norvegicus
160000
-
plus 80000, 40000 and 300000, mutant Y110F Rattus norvegicus
300000
-
above 300000, wild-type and part of mutant Y110F Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NAD+
-
Rattus norvegicus D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Rattus norvegicus ?
-
?
ethanol + NAD+
-
Rattus norvegicus acetaldehyde + NADH
-
?

Subunits

Subunits Comment Organism
dimer plus tetramer and monomer, mutant 110F Rattus norvegicus
monomer plus tetramer and monomer, mutant 110F Rattus norvegicus
More network of coupled hydrogen bonds holds up the protein interface and tetramer stability Rattus norvegicus
tetramer MALDI-TOF, wild-type and part of mutant Y110F Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.63
-
D-xylitol wild-type, pH 10.0 Rattus norvegicus
0.71
-
D-sorbitol wild-type, pH 10.0 Rattus norvegicus