Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5-alpha cells | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0256 | - |
NADP+ | at pH 10.0, temperature not specified in the publication | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54600 | - |
- |
Gluconobacter oxydans |
54900 | - |
- |
Gluconobacter oxydans |
100000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | Gluconobacter oxydans | - |
D-fructose + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol + NADP+ | - |
Gluconobacter oxydans | ? | - |
? | |
D-mannitol + NADP+ | - |
Gluconobacter oxydans | D-fructose + NADPH + H+ | - |
? | |
D-sorbitol + NADP+ | - |
Gluconobacter oxydans | ? | - |
? | |
glycerol + NADP+ | - |
Gluconobacter oxydans | ? | - |
? | |
additional information | the enzyme is able to reduce D-fructose, L-sorbose, and 5-keto D-gluconate in the presence of NADPH as an electron donor | Gluconobacter oxydans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54600, calculated from amino acid sequence | Gluconobacter oxydans |
homodimer | 2 * 54900, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
Gox1432 | - |
Gluconobacter oxydans |
NADP+-dependent mannitol dehydrogenase | - |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Gluconobacter oxydans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is crucial for cell survival under osmotic stress | Gluconobacter oxydans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
D-mannitol | at pH 10.0, temperature not specified in the publication | Gluconobacter oxydans | |
0.66 | - |
D-mannitol | at pH 7.0, temperature not specified in the publication | Gluconobacter oxydans | |
0.89 | - |
D-sorbitol | at pH 10.0, temperature not specified in the publication | Gluconobacter oxydans |