Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activates 5% at 5 mM, reduction reaction | Tuber borchii | |
additional information | the enzyme shows a carbon responsive transcriptional pattern and is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose, overview | Tuber borchii |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, the enzyme shows a carbon responsive transcriptional pattern and is up-regulated when mycelia are transferred to a culture medium containing D-mannitol or D-fructose, quantitative expression and phylogenetic analysis | Tuber borchii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibits 96% at 4 mM, reduction reaction | Tuber borchii | |
2-mercaptoethanol | inhibits 38% at 25 mM, reduction reaction | Tuber borchii | |
Ca2+ | inhibits 21% at 1 mM, reduction reaction | Tuber borchii | |
Cu2+ | inhibits 81% at 0.005 mM, reduction reaction | Tuber borchii | |
D-fructose | inhibition patterns, overview | Tuber borchii | |
D-mannitol | inhibition patterns, overview | Tuber borchii | |
DTT | inhibits 89% at 0.5 mM, reduction reaction | Tuber borchii | |
Mg2+ | inhibits 19% at 1 mM, reduction reaction | Tuber borchii | |
Mn2+ | inhibits 43% at 1 mM, reduction reaction | Tuber borchii | |
additional information | no inhibition by GSH, Fe2+, and EDTA | Tuber borchii | |
NADP+ | inhibition patterns, overview | Tuber borchii | |
NADPH | inhibition patterns, overview | Tuber borchii | |
Zn2+ | inhibits 76% at 0.01 mM, reduction reaction | Tuber borchii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Tuber borchii | |
0.066 | - |
NADP+ | oxidation reaction, pH 9.0, 37°C | Tuber borchii | |
0.1 | - |
NADPH | reduction reaction, pH 7.5, 37°C | Tuber borchii | |
47 | - |
D-fructose | reduction reaction, pH 7.5, 37°C | Tuber borchii | |
600 | - |
D-mannitol | oxidation reaction, pH 9.0, 37°C | Tuber borchii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | two zinc atoms per subunit | Tuber borchii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE, peptide mapping | Tuber borchii |
163000 | - |
gel filtration | Tuber borchii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | Tuber borchii | enzyme regulation mode, overview | D-fructose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tuber borchii | Q1ACW3 | plant symbiotic ascomycete fungus, strain MYA-1019 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from mycelia 368fold by anion exchange chromatography, ultrafiltration, and 2',5'-ADP affinity chromatography | Tuber borchii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-mannitol + NADP+ = D-fructose + NADPH + H+ | sequential ordered bi bi mechanism | Tuber borchii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the fruitbody growing on Pinus pinea roots | Tuber borchii | - |
mycelium | - |
Tuber borchii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
350 | - |
purified native enzyme | Tuber borchii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | - |
Tuber borchii | D-fructose + NADPH + H+ | - |
r | |
D-mannitol + NADP+ | enzyme regulation mode, overview | Tuber borchii | D-fructose + NADPH + H+ | - |
r | |
additional information | the enzyme has a high preference only for D-fructose and D-mannitol, while D-mannose, D-arabinose, D-ribose, D-glucose, D-galactose, D-xylose, D-sorbose and D-sorbitol show only poor activity | Tuber borchii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, SDS-PAGE, peptide mapping | Tuber borchii |
Synonyms | Comment | Organism |
---|---|---|
D-mannitol 2-dehydrogenase | - |
Tuber borchii |
D-mannitol dehydrogenase | - |
Tuber borchii |
More | the enzyme defines a subfamily within the polyol-specific medium chain dehydrogenases | Tuber borchii |
TbMDH | - |
Tuber borchii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | both reaction directions | Tuber borchii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | reduction reaction | Tuber borchii |
9 | - |
oxidation reaction | Tuber borchii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | strictly dependent on NADP(H), no activity with NAD(H) | Tuber borchii | |
NADPH | strictly dependent on NADP(H), no activity with NAD(H) | Tuber borchii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate/product inhibition patterns, overview | Tuber borchii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tuber borchii | 2D-PAGE | - |
5.8 |