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Literature summary for 1.1.1.138 extracted from

  • Bubner, P.; Klimacek, M.; Nidetzky, B.
    Structure-guided engineering of the coenzyme specificity of Pseudomonas fluorescens mannitol 2-dehydrogenase to enable efficient utilization of NAD(H) and NADP(H) (2008), FEBS Lett., 582, 233-237.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes in Escherichia coli strain JM109 Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
D69A site-directed mutagenesis, the mutant shows an altered cofactor specificity compared to the wild-type enzyme, cf. EC 1.1.1.67, which is switched to NADP(H), NADP(H) is equally utilized as NAD(H) Pseudomonas fluorescens
E68K site-directed mutagenesis, the mutant shows an altered cofactor specificity compared to the wild-type enzyme, cf. EC 1.1.1.67, which is switched to NADP(H), NADP(H) is preferred by 10fold over NAD(H) Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic analysis, recombinant mutant enzymes, overview Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol + NADP+ Pseudomonas fluorescens
-
D-fructose + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NADP+
-
Pseudomonas fluorescens D-fructose + NADPH + H+
-
r
D-mannitol + NADP+ only the mutant enzymes D69A amd E68K, the wild-type enzyme utilizes NAD(H) as cofactor, EC 1.1.1.67 Pseudomonas fluorescens D-fructose + NADPH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, both reaction directions Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at, both reaction directions Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Pseudomonas fluorescens
NADPH
-
Pseudomonas fluorescens