Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli strain JM109 | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
D69A | site-directed mutagenesis, the mutant shows an altered cofactor specificity compared to the wild-type enzyme, cf. EC 1.1.1.67, which is switched to NADP(H), NADP(H) is equally utilized as NAD(H) | Pseudomonas fluorescens |
E68K | site-directed mutagenesis, the mutant shows an altered cofactor specificity compared to the wild-type enzyme, cf. EC 1.1.1.67, which is switched to NADP(H), NADP(H) is preferred by 10fold over NAD(H) | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, recombinant mutant enzymes, overview | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | Pseudomonas fluorescens | - |
D-fructose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NADP+ | - |
Pseudomonas fluorescens | D-fructose + NADPH + H+ | - |
r | |
D-mannitol + NADP+ | only the mutant enzymes D69A amd E68K, the wild-type enzyme utilizes NAD(H) as cofactor, EC 1.1.1.67 | Pseudomonas fluorescens | D-fructose + NADPH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, both reaction directions | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at, both reaction directions | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas fluorescens |