Application | Comment | Organism |
---|---|---|
synthesis | commercial mannitol production as alternatives to less efficient chemical reduction of fructose | Agaricus bisporus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agaricus bisporus |
Crystallization (Comment) | Organism |
---|---|
binary complex with NADP+ | Agaricus bisporus |
binary complex with NADP+ at 1.5 A resolution, catalytic triad is assigned to Ser149, Tyr169 and Lys173 | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | Agaricus bisporus | - |
D-mannitol + NADP+ | - |
r | |
D-mannitol + NADP+ | Agaricus bisporus | mannitol serves as storage carbon, plays a critical role in growth, fruit body development, osmoregulation and salt tolerance | D-fructose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Agaricus bisporus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | - |
Agaricus bisporus | D-mannitol + NADP+ | - |
r | |
D-mannitol + NADP+ | mannitol serves as storage carbon, plays a critical role in growth, fruit body development, osmoregulation and salt tolerance | Agaricus bisporus | D-fructose + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | data from X-ray structure | Agaricus bisporus |
tetramer | crystallization data, residues Ser149, Tyr169, Lys173 form a catalytic triad | Agaricus bisporus |
Synonyms | Comment | Organism |
---|---|---|
MtDH | - |
Agaricus bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for NADP+ | Agaricus bisporus | |
NADPH | - |
Agaricus bisporus |