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Literature summary for 1.1.1.138 extracted from

  • Adachi, O.; Toyama, H.; Matsushita, K.
    Crystalline NADP-dependent D-mannitol dehydrogenase from Gluconobacter suboxydans (1999), Biosci. Biotechnol. Biochem., 63, 402-407.
    View publication on PubMed

Application

Application Comment Organism
analysis determination of D-fructose in the presence of other sugars Gluconobacter oxydans

Crystallization (Commentary)

Crystallization (Comment) Organism
ammonium sulfate precipitation Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
NADPH
-
Gluconobacter oxydans
0.025
-
NADP+
-
Gluconobacter oxydans
10
-
D-mannitol
-
Gluconobacter oxydans
12
-
D-fructose
-
Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose + NADPH Gluconobacter oxydans
-
D-mannitol + NADP+
-
?
D-fructose + NADPH Gluconobacter oxydans IFO 12528
-
D-mannitol + NADP+
-
?
D-mannitol + NADP+ Gluconobacter oxydans
-
D-fructose + NADPH + H+
-
r
D-mannitol + NADP+ Gluconobacter oxydans IFO 12528
-
D-fructose + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans IFO 12528
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to crystalline state, chromatography techniques, crystallization Gluconobacter oxydans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Gluconobacter oxydans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
235
-
-
Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADPH
-
Gluconobacter oxydans D-mannitol + NADP+
-
?
D-fructose + NADPH
-
Gluconobacter oxydans IFO 12528 D-mannitol + NADP+
-
?
D-mannitol + NADP+
-
Gluconobacter oxydans D-fructose + NADPH + H+
-
r
D-mannitol + NADP+
-
Gluconobacter oxydans IFO 12528 D-fructose + NADPH + H+
-
r
L-sorbitol + NADP+ 4% of the activity compared to D-mannitol Gluconobacter oxydans L-sorbose + NADPH
-
r
L-sorbitol + NADP+ 4% of the activity compared to D-mannitol Gluconobacter oxydans IFO 12528 L-sorbose + NADPH
-
r

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
D-mannitol dehydrogenase, NADP-dependent
-
Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable over 30 min Gluconobacter oxydans
53
-
20-50% retaining activity after 20 min Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 reduction of D-fructose Gluconobacter oxydans
9 10 oxidation of D-mannitol Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9.5 stable within Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for NADP+ Gluconobacter oxydans
NADPH
-
Gluconobacter oxydans