Literature summary for 1.1.1.136 extracted from

Miller, W.L.; Wenzel, C.Q.; Daniels, C.; Larocque, S.; Brisson, J.R.; Lam, J.S.
Biochemical characterization of WbpA, a UDP-N-acetyl-D-glucosamine 6-dehydrogenase involved in O-antigen biosynthesis in Pseudomonas aeruginosa PAO1 (2004), J. Biol. Chem., 279, 37551-37558.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	tetramethylammonium, dimethylammonium or sodium slats do not substitute for NH4+ or K+	Pseudomonas aeruginosa
NH4+	or K+, required	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
-	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	positive cooperativity with respect to substrate binding, Hill coefficient 1.8	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	or NH4+, required	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50300	-	3 * 50300, SDS-PAGE	Pseudomonas aeruginosa
153000	-	dynamic light scattering	Pseudomonas aeruginosa
162000	-	gel filtration	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein with His-tag	Pseudomonas aeruginosa

Storage Stability

Storage Stability	Organism
-20°C, 25% glycerol, stable for months	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O	-	Pseudomonas aeruginosa	UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+	-	?

Subunits

Subunits	Comment	Organism
trimer	3 * 50300, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
WbpA	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
33	37	-	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	9	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas aeruginosa

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas aeruginosa	isoelectric focusing	-	5.5
Pseudomonas aeruginosa	predicted from gene sequence	-	6

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Release 2023.1 (January 2023)