Literature summary for 1.1.1.136 extracted from

Kawamura, T.; Ichihara, N.; Sugiyama, S.; Yokota, H.; Ishimoto, N.; Ito, E.
Biosynthesis of UDP-N-acetyl-D-glucosaminuronic acid and UDP-N-acetyl-D-mannosaminuronic acid in Micrococcus luteus (1985), J. Biochem., 98, 105-116.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	and the particulate fraction required for full activity	Micrococcus luteus

General Stability

General Stability	Organism
ammonium sulfate protects against inactivation during storage and incubation	Micrococcus luteus
monovalent cations protect against inactivation during incubation	Micrococcus luteus

Inhibitors

Inhibitors	Comment	Organism	Structure
ethanol	10 mM, more than 90% inhibition	Micrococcus luteus
N-acetylglucosamine 1-phosphate	-	Micrococcus luteus
p-chloromercuribenzoate	0.027 mM, complete inhibition	Micrococcus luteus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.28	-	UDP-N-acetyl-D-glucosamine	-	Micrococcus luteus
1.43	-	NAD+	-	Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O	Micrococcus luteus	-	UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Micrococcus luteus	-	ATCC 4698	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
41	-	-	Micrococcus luteus

Storage Stability

Storage Stability	Organism
4°C, 1 M (NH4)2SO4, stable for more than 3 weeks	Micrococcus luteus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O	-	Micrococcus luteus	UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.8	-	above, Tris buffer	Micrococcus luteus
10	-	above, glycine-NaOH buffer	Micrococcus luteus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Micrococcus luteus

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Release 2023.1 (January 2023)