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Literature summary for 1.1.1.133 extracted from
- Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar typhimurium LT2 (1999), J. Biol. Chem., 274, 25069-25077.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli BL21(lambdaDE3) | Salmonella enterica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | up to 0.3 mM, 70% loss of enzyme activity | Salmonella enterica |  |
| additional information | full activity restored with up to 0.3 mM MgCl2 | Salmonella enterica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.106 | - |
NADH | +/-0.018, pH 7.0 | Salmonella enterica | |
| 0.21 | - |
NADH | +/-0.004, pH 7.0 | Salmonella enterica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32600 | - |
calculated from nucleotide sequence data | Salmonella enterica |
| 34800 | - |
SDS-PAGE | Salmonella enterica |
| 41500 | - |
gel filtration | Salmonella enterica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
serovar typhimurium LT2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity and ion exchange chromatography | Salmonella enterica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44 | - |
- |
Salmonella enterica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 44% glycerol | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+ | - |
Salmonella enterica | dTDP-beta-L-rhamnose + NAD+ | - |
? | |
| dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H | - |
Salmonella enterica | dTDP-L-rhamnose + NAD(P)+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dTDP-4-dehydrorhamnose reductase | - |
Salmonella enterica |
| thymidine diphosphate-4-dehydrorhamnose reductase | - |
Salmonella enterica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below | Salmonella enterica |
| 37 | - |
pH 7.0, 75% activity loss | Salmonella enterica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25 | - |
NADH | +/-48 | Salmonella enterica | |
| 29 | - |
NADPH | +/-66 | Salmonella enterica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
reduction of dTDP-6-deoxy-L-lyxo-4-hexulose | Salmonella enterica |
| 10 | - |
oxidation of dTDP-L-rhamnose | Salmonella enterica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
37°C, 20 min, 75% activity loss | Salmonella enterica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Salmonella enterica | |
| NADH | preferred | Salmonella enterica | |
| NADP+ | - |
Salmonella enterica | |
| NADPH | - |
Salmonella enterica | |
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